Protein Info for Rv0685 in Mycobacterium tuberculosis H37Rv

Annotation: Probable iron-regulated elongation factor TU Tuf (EF-TU)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 396 TIGR00485: translation elongation factor Tu" amino acids 2 to 396 (395 residues), 715.6 bits, see alignment E=1.3e-219 PF00009: GTP_EFTU" amino acids 10 to 202 (193 residues), 217.1 bits, see alignment E=2.3e-68 TIGR00231: small GTP-binding protein domain" amino acids 13 to 156 (144 residues), 50.2 bits, see alignment E=2.6e-17 PF03144: GTP_EFTU_D2" amino acids 226 to 296 (71 residues), 71.5 bits, see alignment E=9.5e-24 PF03143: GTP_EFTU_D3" amino acids 301 to 394 (94 residues), 133.9 bits, see alignment E=4e-43

Best Hits

Swiss-Prot: 100% identical to EFTU_MYCBT: Elongation factor Tu (tuf) from Mycobacterium bovis (strain BCG / Tokyo 172 / ATCC 35737 / TMC 1019)

KEGG orthology group: K02358, elongation factor Tu (inferred from 100% identity to mtu:Rv0685)

Predicted SEED Role

"Translation elongation factor Tu" in subsystem Universal GTPases

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (396 amino acids)

>Rv0685 Probable iron-regulated elongation factor TU Tuf (EF-TU) (Mycobacterium tuberculosis H37Rv)
VAKAKFQRTKPHVNIGTIGHVDHGKTTLTAAITKVLHDKFPDLNETKAFDQIDNAPEERQ
RGITINIAHVEYQTDKRHYAHVDAPGHADYIKNMITGAAQMDGAILVVAATDGPMPQTRE
HVLLARQVGVPYILVALNKADAVDDEELLELVEMEVRELLAAQEFDEDAPVVRVSALKAL
EGDAKWVASVEELMNAVDESIPDPVRETDKPFLMPVEDVFTITGRGTVVTGRVERGVINV
NEEVEIVGIRPSTTKTTVTGVEMFRKLLDQGQAGDNVGLLLRGVKREDVERGQVVTKPGT
TTPHTEFEGQVYILSKDEGGRHTPFFNNYRPQFYFRTTDVTGVVTLPEGTEMVMPGDNTN
ISVKLIQPVAMDEGLRFAIREGGRTVGAGRVTKIIK