Protein Info for Rv0470c in Mycobacterium tuberculosis H37Rv

Annotation: Mycolic acid synthase PcaA (cyclopropane synthase)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 287 PF02353: CMAS" amino acids 5 to 283 (279 residues), 397 bits, see alignment E=1.1e-122 PF13489: Methyltransf_23" amino acids 51 to 224 (174 residues), 40 bits, see alignment E=8.5e-14 PF08241: Methyltransf_11" amino acids 69 to 165 (97 residues), 26.5 bits, see alignment E=2.1e-09 PF13649: Methyltransf_25" amino acids 69 to 162 (94 residues), 37.6 bits, see alignment E=7.6e-13

Best Hits

Swiss-Prot: 100% identical to CMAS3_MYCTO: Cyclopropane mycolic acid synthase 3 (pcaA) from Mycobacterium tuberculosis (strain CDC 1551 / Oshkosh)

KEGG orthology group: K00574, cyclopropane-fatty-acyl-phospholipid synthase [EC: 2.1.1.79] (inferred from 100% identity to mbt:JTY_0480)

MetaCyc: 73% identical to methoxy mycolic acid synthase 2 (Mycobacterium tuberculosis H37Rv)
Cyclopropane-fatty-acyl-phospholipid synthase. [EC: 2.1.1.79]; 2.1.1.79 [EC: 2.1.1.79]; 2.1.1.79 [EC: 2.1.1.79]; 2.1.1.79 [EC: 2.1.1.79]

Predicted SEED Role

No annotation

MetaCyc Pathways

Isozymes

Compare fitness of predicted isozymes for: 2.1.1.79

Use Curated BLAST to search for 2.1.1.79

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (287 amino acids)

>Rv0470c Mycolic acid synthase PcaA (cyclopropane synthase) (Mycobacterium tuberculosis H37Rv)
MSVQLTPHFGNVQAHYDLSDDFFRLFLDPTQTYSCAYFERDDMTLQEAQIAKIDLALGKL
NLEPGMTLLDIGCGWGATMRRAIEKYDVNVVGLTLSENQAGHVQKMFDQMDTPRSRRVLL
EGWEKFDEPVDRIVSIGAFEHFGHQRYHHFFEVTHRTLPADGKMLLHTIVRPTFKEGREK
GLTLTHELVHFTKFILAEIFPGGWLPSIPTVHEYAEKVGFRVTAVQSLQLHYARTLDMWA
TALEANKDQAIAIQSQTVYDRYMKYLTGCAKLFRQGYTDVDQFTLEK