Protein Info for Rv0468 in Mycobacterium tuberculosis H37Rv

Annotation: 3-hydroxybutyryl-CoA dehydrogenase FadB2 (beta-hydroxybutyryl-CoA dehydrogenase) (BHBD)

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02737: 3HCDH_N
  • amino acids 8 to 187 (180 residues), 201.2 bits, E=2e-63
• PF00725: 3HCDH
  • amino acids 190 to 286 (97 residues), 116.1 bits, E=1.2e-37

Best Hits

Swiss-Prot: 100% identical to FADB2_MYCTU: 3-hydroxybutyryl-CoA dehydrogenase (fadB2) from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

KEGG orthology group: K00074, 3-hydroxybutyryl-CoA dehydrogenase [EC: 1.1.1.157] (inferred from 99% identity to mtb:TBMG_00470)

MetaCyc: 43% identical to 3-hydroxybutyryl-CoA dehydrogenase subunit (Clostridium beijerinckii)
3-hydroxyacyl-CoA dehydrogenase. [EC: 1.1.1.35]; 1.1.1.35 [EC: 1.1.1.35]

Predicted SEED Role

"3-hydroxybutyryl-CoA dehydrogenase (EC 1.1.1.157); 3-hydroxyacyl-CoA dehydrogenase (EC 1.1.1.35)" (EC 1.1.1.157, EC 1.1.1.35)

MetaCyc Pathways

• androstenedione degradation I (aerobic) (22/25 steps found)
• superpathway of cholesterol degradation I (cholesterol oxidase) (34/42 steps found)
• superpathway of cholesterol degradation II (cholesterol dehydrogenase) (36/47 steps found)
• superpathway of testosterone and androsterone degradation (22/28 steps found)
• oleate β-oxidation (27/35 steps found)
• benzoyl-CoA biosynthesis (3/3 steps found)
• fatty acid salvage (5/6 steps found)
• adipate biosynthesis (4/5 steps found)
• adipate degradation (4/5 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (10/14 steps found)
• cholesterol degradation to androstenedione I (cholesterol oxidase) (12/17 steps found)
• pyruvate fermentation to butanol II (engineered) (4/6 steps found)
• superpathway of Clostridium acetobutylicum acidogenic fermentation (6/9 steps found)
• valproate β-oxidation (6/9 steps found)
• fatty acid β-oxidation II (plant peroxisome) (3/5 steps found)
• glutaryl-CoA degradation (3/5 steps found)
• pyruvate fermentation to hexanol (engineered) (7/11 steps found)
• fatty acid β-oxidation I (generic) (4/7 steps found)
• pyruvate fermentation to butanoate (4/7 steps found)
• 2-methyl-branched fatty acid β-oxidation (9/14 steps found)
• methyl ketone biosynthesis (engineered) (3/6 steps found)
• glycerol degradation to butanol (10/16 steps found)
• (R)- and (S)-3-hydroxybutanoate biosynthesis (engineered) (2/5 steps found)
• 4-hydroxybenzoate biosynthesis III (plants) (2/5 steps found)
• pyruvate fermentation to butanol I (4/8 steps found)
• cholesterol degradation to androstenedione II (cholesterol dehydrogenase) (14/22 steps found)
• fatty acid β-oxidation VI (mammalian peroxisome) (3/7 steps found)
• L-glutamate degradation V (via hydroxyglutarate) (5/10 steps found)
• 1-butanol autotrophic biosynthesis (engineered) (17/27 steps found)
• 3-hydroxypropanoate/4-hydroxybutanate cycle (10/18 steps found)
• benzoyl-CoA degradation I (aerobic) (2/7 steps found)
• superpathway of Clostridium acetobutylicum solventogenic fermentation (6/13 steps found)
• benzoate biosynthesis I (CoA-dependent, β-oxidative) (3/9 steps found)
• photosynthetic 3-hydroxybutanoate biosynthesis (engineered) (15/26 steps found)
• 2-methylpropene degradation (2/8 steps found)
• superpathway of phenylethylamine degradation (4/11 steps found)
• 3-phenylpropanoate degradation (3/10 steps found)
• superpathway of Clostridium acetobutylicum acidogenic and solventogenic fermentation (8/17 steps found)
• phenylacetate degradation I (aerobic) (2/9 steps found)
• L-glutamate degradation VII (to butanoate) (4/12 steps found)
• methyl tert-butyl ether degradation (2/10 steps found)
• androstenedione degradation II (anaerobic) (13/27 steps found)
• L-tryptophan degradation III (eukaryotic) (4/15 steps found)
• crotonate fermentation (to acetate and cyclohexane carboxylate) (3/16 steps found)
• benzoate fermentation (to acetate and cyclohexane carboxylate) (3/17 steps found)
• toluene degradation VI (anaerobic) (3/18 steps found)
• platensimycin biosynthesis (7/26 steps found)
• superpathway of cholesterol degradation III (oxidase) (23/49 steps found)

KEGG Metabolic Maps

• Benzoate degradation via CoA ligation
• Bile acid biosynthesis
• Biosynthesis of plant hormones
• Butanoate metabolism
• Caprolactam degradation
• Fatty acid elongation in mitochondria
• Fatty acid metabolism
• Geraniol degradation
• Lysine degradation
• Tryptophan metabolism
• Valine, leucine and isoleucine degradation

Isozymes

Compare fitness of predicted isozymes for: 1.1.1.157, 1.1.1.35

Use Curated BLAST to search for 1.1.1.157 or 1.1.1.35

Protein Sequence (286 amino acids)

>Rv0468 3-hydroxybutyryl-CoA dehydrogenase FadB2 (beta-hydroxybutyryl-CoA dehydrogenase) (BHBD) (Mycobacterium tuberculosis H37Rv)
VSDAIQRVGVVGAGQMGSGIAEVSARAGVEVTVFEPAEALITAGRNRIVKSLERAVSAGK
VTERERDRALGLLTFTTDLNDLSDRQLVIEAVVEDEAVKSEIFAELDRVVTDPDAVLASN
TSSIPIMKVAAATKQPQRVLGLHFFNPVPVLPLVELVRTLVTDEAAAARTEEFASTVLGK
QVVRCSDRSGFVVNALLVPYLLSAIRMVEAGFATVEDVDKAVVAGLSHPMGPLRLSDLVG
LDTLKLIADKMFEEFKEPHYGPPPLLLRMVEAGQLGKKSGRGFYTY