Protein Info for Rv0415 in Mycobacterium tuberculosis H37Rv

Annotation: Possible thiamine biosynthesis oxidoreductase ThiO

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01266: DAO
  • amino acids 10 to 329 (320 residues), 188.3 bits, E=1.5e-59
• TIGR02352: glycine oxidase ThiO
  • amino acids 11 to 334 (324 residues), 364.8 bits, E=2.3e-113

Best Hits

KEGG orthology group: K03153, glycine oxidase [EC: 1.4.3.19] (inferred from 100% identity to mtb:TBMG_00416)

Predicted SEED Role

"Glycine oxidase ThiO (EC 1.4.3.19)" in subsystem Thiamin biosynthesis (EC 1.4.3.19)

MetaCyc Pathways

• superpathway of thiamine diphosphate biosynthesis II (10/11 steps found)
• thiazole component of thiamine diphosphate biosynthesis II (6/7 steps found)
• glyphosate degradation II (1/1 steps found)
• butanol and isobutanol biosynthesis (engineered) (4/8 steps found)

KEGG Metabolic Maps

• Thiamine metabolism

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.4.3.19

Protein Sequence (340 amino acids)

>Rv0415 Possible thiamine biosynthesis oxidoreductase ThiO (Mycobacterium tuberculosis H37Rv)
MASDLHTGSLAVIGGGVIGLSVARRAAQAGWPVRVHRSDERGASWVAGGMLAPHSEGWPG
EERLLRLGLQSLRLWREGSFLDGLGPQLVTAHESLVVAVDRADVADLRTVADWLSAQGHP
VIWESAARDVEPLLAQGIRHGFRAPTELAVDNRALLDALCRDCERLGVRWSSQVSSLSDV
DAHTVVIANGIDAPALWPGLPIRPVKGEVLRLRWRPGCMPLPQRVIRARVRGRQVYLVPR
SDGVVVGATQYEHGRDTAPVVSGVRDLLDDACTVLPALGEYELAECEAGLRPMTPDNLPL
VQRLDSRTLVAAGHGRSGFLLAPWTAEQIVSELVSVGAAS