Protein Info for Rv0252 in Mycobacterium tuberculosis H37Rv

Annotation: Probable nitrite reductase [NAD(P)H] large subunit [FAD flavoprotein] NirB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 853 PF07992: Pyr_redox_2" amino acids 15 to 302 (288 residues), 173.6 bits, see alignment E=2.6e-54 TIGR02374: nitrite reductase [NAD(P)H], large subunit" amino acids 16 to 812 (797 residues), 1315.7 bits, see alignment E=0 PF00070: Pyr_redox" amino acids 165 to 244 (80 residues), 58.2 bits, see alignment E=3.8e-19 PF18267: Rubredoxin_C" amino acids 339 to 399 (61 residues), 34.3 bits, see alignment 7.4e-12 PF04324: Fer2_BFD" amino acids 441 to 488 (48 residues), 40.2 bits, see alignment 1.4e-13 PF03460: NIR_SIR_ferr" amino acids 573 to 633 (61 residues), 37.7 bits, see alignment 5.4e-13 PF01077: NIR_SIR" amino acids 645 to 782 (138 residues), 90.3 bits, see alignment E=3.7e-29

Best Hits

KEGG orthology group: K00362, nitrite reductase (NAD(P)H) large subunit [EC: 1.7.1.4] (inferred from 100% identity to mtb:TBMG_00254)

Predicted SEED Role

"Nitrite reductase [NAD(P)H] large subunit (EC 1.7.1.4)" in subsystem Nitrate and nitrite ammonification (EC 1.7.1.4)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.7.1.4

Use Curated BLAST to search for 1.7.1.4

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (853 amino acids)

>Rv0252 Probable nitrite reductase [NAD(P)H] large subunit [FAD flavoprotein] NirB (Mycobacterium tuberculosis H37Rv)
MPTAGSSRAPAAAREIVVVGHGMVGHRLVEAVRARDADGSLRITVLAEEGDAAYDRVGLT
SYTESWDRALLALPGNDYAGDQRVRLLLNTRVTQIDRATKSVVTAAGQRHRYDTLVLATG
SYAFVPPVPGHDLPACHVYRTFDDLDAIRAGAQRTLDGGHTDGGVVIGGGLLGLEAANAL
RQFGLQTHVVEMMPRLMAQQIDEAGGALLARMIADLGIAVHVGTGTESIESVKHSDGSVW
ARVRLSDGEVIDAGVVIFAAGIRPRDELARAAGLAIGDRGGVLTDLSCRTSDPDIYAVGE
VAAIDGRCYGLVGPGYTSAEVVADRLLDGSAEFPEADLSTKLKLLGVDVASFGDAMGATE
NCLEVVINDAVKRTYAKLVLSDDATTLLGGVLVGDASSYGVLRPMVGAELPGDPLALIAP
AGSGAGAGALGVGALPDSAQICSCNNVTKGELKCAIADGCGDVPALKSCTAAGTSCGSCV
PLLKQLLEAEGVEQSKALCEHFSQSRAELFEIITATEVRTFSGLLDRFGRGKGCDICKPV
VASILASTGSDHILDGEQASLQDSNDHFLANIQKNGSYSVVPRVPGGDIKPEHLILIGQI
AQDFGLYTKITGGQRIDLFGARVDQLPLIWQRLVDGGMESGHAYGKAVRTVKSCVGSDWC
RYGQQDSVQLAIDLELRYRGLRAPHKIKLGVSGCARECAEARGKDVGVIATEKGWNLYVA
GNGGMTPKHAQLLASDLDKETLIRYIDRFLIYYIRTADRLQRTAPWVESLGLDHVREVVC
EDSLGLAEEFEAAMQRHVANYKCEWKGVLEDPDKLSRFVSFVNAPDAVDSTVTFTERAGR
KVPVSIGIPRVRS