Protein Info for Rv0157 in Mycobacterium tuberculosis H37Rv
Annotation: Probable NAD(P) transhydrogenase (subunit beta) PntB [integral membrane protein] (pyridine nucleotide transhydrogenase subunit beta) (nicotinamide nucleotide transhydrogenase subunit beta)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K00325, NAD(P) transhydrogenase subunit beta [EC: 1.6.1.2] (inferred from 100% identity to mbo:Mb0162)Predicted SEED Role
"NAD(P) transhydrogenase subunit beta (EC 1.6.1.2)" in subsystem Phosphate metabolism (EC 1.6.1.2)
MetaCyc Pathways
- NAD(P)/NADPH interconversion (3/6 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 1.6.1.2
Use Curated BLAST to search for 1.6.1.2
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (475 amino acids)
>Rv0157 Probable NAD(P) transhydrogenase (subunit beta) PntB [integral membrane protein] (pyridine nucleotide transhydrogenase subunit beta) (nicotinamide nucleotide transhydrogenase subunit beta) (Mycobacterium tuberculosis H37Rv) MNLHYLVEILYIISFSLFIYGLMGLTGPKTAVRGNLIAAAGMTIAVAATLVMIRHTSQWP LIIAGLVVGVVLGVPPARLTKMTAMPQLVAFFNGVGGGTVALIALSEFIDTTGFSAFQHG ESPTVHIVVASLFAAIIGSISFWGSIVAFGKLQEIISGRPIGLGKAQQPINLLLLAVAVA AAVVIGLHAHPGSGGVALWWMIGLLVAAGVLGLMVVLPIGGADMPVVISMLNAMTGLSAA AAGLALNNTAMIVAGMIVGASGSILTNLMAKAMNRSIPAIVAGGFGGGGVAPSGGGDDKH VKATSAADAAIQMAYANQVIVVPGYGLAVAQAQHAVKDLATLLEDRGVPVKYAIHPVAGR MPGHMNVLLAEAEVDYDAMKDMDDINDEFARTDVTIVIGANDVTNPAARNETSSPIYGMP ILNVDKSRSVIVLKRSMNSGFAGIDNPLFYADGTTMLFGDAKKSVTEVSEELKAL