Protein Info for Rv0114 in Mycobacterium tuberculosis H37Rv
Annotation: Possible D-alpha,beta-D-heptose-1,7-biphosphate phosphatase GmhB (D-glycero-D-manno-heptose 7-phosphate kinase)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to GMHBA_MYCTU: D-glycero-alpha-D-manno-heptose-1,7-bisphosphate 7-phosphatase (gmhB) from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
KEGG orthology group: K03273, D-glycero-D-manno-heptose 1,7-bisphosphate phosphatase [EC: 3.1.3.-] (inferred from 100% identity to mbo:Mb0118)Predicted SEED Role
"Phosphoheptose isomerase (EC 5.3.1.-)" in subsystem Capsular heptose biosynthesis or LOS core oligosaccharide biosynthesis (EC 5.3.1.-)
MetaCyc Pathways
- GDP-D-glycero-α-D-manno-heptose biosynthesis (1/4 steps found)
- ADP-L-glycero-β-D-manno-heptose biosynthesis (1/5 steps found)
KEGG Metabolic Maps
- Aminosugars metabolism
- Ascorbate and aldarate metabolism
- Biosynthesis of ansamycins
- Ether lipid metabolism
- Fructose and mannose metabolism
- Galactose metabolism
- Inositol phosphate metabolism
- Lipopolysaccharide biosynthesis
- Nicotinate and nicotinamide metabolism
- Pentose and glucuronate interconversions
- Riboflavin metabolism
- Sphingolipid metabolism
- Thiamine metabolism
Isozymes
Compare fitness of predicted isozymes for: 3.1.3.-, 5.3.1.-
Use Curated BLAST to search for 3.1.3.- or 5.3.1.-
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (190 amino acids)
>Rv0114 Possible D-alpha,beta-D-heptose-1,7-biphosphate phosphatase GmhB (D-glycero-D-manno-heptose 7-phosphate kinase) (Mycobacterium tuberculosis H37Rv) MVAERAGHQWCLFLDRDGVINRQVVGDYVRNWRQFEWLPGAARALKKLRAWAPYIVVVTN QQGVGAGLMSAVDVMVIHRHLQMQLASDGVLIDGFQVCPHHRSQRCGCRKPRPGLVLDWL GRHPDSEPLLSIVVGDSLSDLELAHNVAAAAGACASVQIGGASSGGVADASFDSLWEFAV AVGHARGERG