Protein Info for Rv0103c in Mycobacterium tuberculosis H37Rv

Annotation: Probable cation-transporter P-type ATPase B CtpB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 752 transmembrane" amino acids 105 to 126 (22 residues), see Phobius details amino acids 132 to 153 (22 residues), see Phobius details amino acids 169 to 188 (20 residues), see Phobius details amino acids 208 to 226 (19 residues), see Phobius details amino acids 361 to 383 (23 residues), see Phobius details amino acids 392 to 414 (23 residues), see Phobius details amino acids 703 to 721 (19 residues), see Phobius details amino acids 726 to 745 (20 residues), see Phobius details PF00403: HMA" amino acids 19 to 76 (58 residues), 36.5 bits, see alignment 1.1e-12 TIGR01525: heavy metal translocating P-type ATPase" amino acids 167 to 746 (580 residues), 544.8 bits, see alignment E=5.8e-167 TIGR01511: copper-translocating P-type ATPase" amino acids 208 to 747 (540 residues), 474.2 bits, see alignment E=1.3e-145 TIGR01494: HAD ATPase, P-type, family IC" amino acids 217 to 480 (264 residues), 121.4 bits, see alignment E=7.3e-39 amino acids 552 to 724 (173 residues), 127.4 bits, see alignment E=1.1e-40 PF00122: E1-E2_ATPase" amino acids 248 to 423 (176 residues), 155.3 bits, see alignment E=2.6e-49 PF00702: Hydrolase" amino acids 440 to 658 (219 residues), 119.1 bits, see alignment E=6.7e-38

Best Hits

Swiss-Prot: 100% identical to CTPB_MYCTU: Cation-transporting P-type ATPase B (ctpB) from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

KEGG orthology group: K12949, cation-transporting P-type ATPase A/B [EC: 3.6.3.-] (inferred from 100% identity to mbo:Mb0106c)

Predicted SEED Role

"Lead, cadmium, zinc and mercury transporting ATPase (EC 3.6.3.3) (EC 3.6.3.5); Copper-translocating P-type ATPase (EC 3.6.3.4)" (EC 3.6.3.3, EC 3.6.3.4, EC 3.6.3.5)

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.-, 3.6.3.3, 3.6.3.4, 3.6.3.5

Use Curated BLAST to search for 3.6.3.- or 3.6.3.3 or 3.6.3.4 or 3.6.3.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (752 amino acids)

>Rv0103c Probable cation-transporter P-type ATPase B CtpB (Mycobacterium tuberculosis H37Rv)
VAAPVVGDADLQSVRRIRLDVLGMSCAACASRVETKLNKIPGVRASVNFATRVATIDAVG
MAADELCGVVEKAGYHAAPHTETTVLDKRTKDPDGAHARRLLRRLLVAAVLFVPLADLST
LFAIVPSARVPGWGYILTALAAPVVTWAAWPFHSVALRNARHRTTSMETLISVGIVAATA
WSLSSVFGDQPPREGSGIWRAILNSDSIYLEVAAGVTVFVLAGRYFEARAKSKAGSALRA
LAELGAKNVAVLLPDGAELVIPASELKKRQRFVTRPGETIAADGVVVDGSAAIDMSAMTG
EAKPVRAYPAASVVGGTVVMDGRLVIEATAVGADTQFAAMVRLVEQAQTQKARAQRLADH
IAGVFVPVVFVIAGLAGAAWLVSGAGADRAFSVTLGVLVIACPCALGLATPTAMMVASGR
GAQLGIFIKGYRALETIRSIDTVVFDKTGTLTVGQLAVSTVTMAGSGTSERDREEVLGLA
AAVESASEHAMAAAIVAASPDPGPVNGFVAVAGCGVSGEVGGHHVEVGKPSWITRTTPCH
DAALVSARLDGESRGETVVFVSVDGVVRAALTIADTLKDSAAAAVAALRSRGLRTILLTG
DNRAAADAVAAQVGIDSAVADMLPEGKVDVIQRLREEGHTVAMVGDGINDGPALVGADLG
LAIGRGTDVALGAADIILVRDDLNTVPQALDLARATMRTIRMNMIWAFGYNVAAIPIAAA
GLLNPLIAGAAMAFSSFFVVSNSLRLRNFGAQ