Protein Info for Rv0058 in Mycobacterium tuberculosis H37Rv

Annotation: Probable replicative DNA helicase DnaB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 874 PF00772: DnaB" amino acids 23 to 125 (103 residues), 116.6 bits, see alignment E=1.2e-37 TIGR00665: replicative DNA helicase" amino acids 24 to 873 (850 residues), 508.5 bits, see alignment E=2.3e-156 PF03796: DnaB_C" amino acids 201 to 871 (671 residues), 315.6 bits, see alignment E=6e-98 PF13481: AAA_25" amino acids 201 to 379 (179 residues), 58.7 bits, see alignment E=1.5e-19 TIGR01445: intein N-terminal splicing region" amino acids 400 to 475 (76 residues), 40.4 bits, see alignment E=7e-14 PF14890: Intein_splicing" amino acids 410 to 815 (406 residues), 65.6 bits, see alignment E=1.1e-21 PF14528: LAGLIDADG_3" amino acids 611 to 694 (84 residues), 73.7 bits, see alignment E=2.9e-24 TIGR01443: intein C-terminal splicing region" amino acids 794 to 816 (23 residues), 27.8 bits, see alignment (E = 3.2e-10)

Best Hits

Swiss-Prot: 100% identical to DNAB_MYCTU: Replicative DNA helicase (dnaB) from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)

KEGG orthology group: K02314, replicative DNA helicase [EC: 3.6.4.12] (inferred from 100% identity to mbo:Mb0059)

Predicted SEED Role

"Replicative DNA helicase (EC 3.6.1.-) @ intein-containing" (EC 3.6.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.-, 3.6.4.12

Use Curated BLAST to search for 3.6.1.- or 3.6.4.12

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Compare to protein structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

Find the best match in UniProt

Protein Sequence (874 amino acids)

>Rv0058 Probable replicative DNA helicase DnaB (Mycobacterium tuberculosis H37Rv)
MAVVDDLAPGMDSSPPSEDYGRQPPQDLAAEQSVLGGMLLSKDAIADVLERLRPGDFYRP
AHQNVYDAILDLYGRGEPADAVTVAAELDRRGLLRRIGGAPYLHTLISTVPTAANAGYYA
SIVAEKALLRRLVEAGTRVVQYGYAGAEGADVAEVVDRAQAEIYDVADRRLSEDFVALED
LLQPTMDEIDAIASSGGLARGVATGFTELDEVTNGLHPGQMVIVAARPGVGKSTLGLDFM
RSCSIRHRMASVIFSLEMSKSEIVMRLLSAEAKIKLSDMRSGRMSDDDWTRLARRMSEIS
EAPLFIDDSPNLTMMEIRAKARRLRQKANLKLIVVDYLQLMTSGKKYESRQVEVSEFSRH
LKLLAKELEVPVVAISQLNRGPEQRTDKKPMLADLRESGCLTASTRILRADTGAEVAFGE
LMRSGERPMVWSLDERLRMVARPMINVFPSGRKEVFRLRLASGREVEATGSHPFMKFEGW
TPLAQLKVGDRIAAPRRVPEPIDTQRMPESELISLARMIGDGSCLKNQPIRYEPVDEANL
AAVTVSAAHSDRAAIRDDYLAARVPSLRPARQRLPRGRCTPIAAWLAGLGLFTKRSHEKC
VPEAVFRAPNDQVALFLRHLWSAGGSVRWDPTNGQGRVYYGSTSRRLIDDVAQLLLRVGI
FSWITHAPKLGGHDSWRLHIHGAKDQVRFLRHVGVHGAEAVAAQEMLRQLKGPVRNPNLD
SAPKKVWAQVRNRLSAKQMMDIQLHEPTMWKHSPSRSRPHRAEARIEDRAIHELARGDAY
WDTVVEITSIGDQHVFDGTVSGTHNFVANGISLHNSLEQDADVVILLHRPDAFDRDDPRG
GEADFILAKHRNGPTKTVTVAHQLHLSRFANMAR