Protein Info for Rv0050 in Mycobacterium tuberculosis H37Rv
Annotation: Probable bifunctional penicillin-binding protein 1A/1B PonA1 (murein polymerase) (PBP1): penicillin-insensitive transglycosylase (peptidoglycan TGASE) + penicillin-sensitive transpeptidase (DD-transpeptidase)
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 100% identical to PBP1A_MYCTU: Penicillin-binding protein 1A (ponA1) from Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv)
KEGG orthology group: None (inferred from 100% identity to mtc:MT0056)Predicted SEED Role
"Multimodular transpeptidase-transglycosylase (EC 2.4.1.129) (EC 3.4.-.-)" in subsystem Peptidoglycan Biosynthesis or Type IV pilus (EC 2.4.1.129, EC 3.4.-.-)
MetaCyc Pathways
- peptidoglycan biosynthesis III (mycobacteria) (14/15 steps found)
- peptidoglycan biosynthesis I (meso-diaminopimelate containing) (11/12 steps found)
- peptidoglycan biosynthesis II (staphylococci) (12/17 steps found)
- peptidoglycan biosynthesis IV (Enterococcus faecium) (12/17 steps found)
- peptidoglycan biosynthesis V (β-lactam resistance) (11/17 steps found)
- peptidoglycan maturation (meso-diaminopimelate containing) (3/12 steps found)
KEGG Metabolic Maps
Isozymes
Compare fitness of predicted isozymes for: 2.4.1.129, 3.4.-.-
Use Curated BLAST to search for 2.4.1.129 or 3.4.-.-
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
Find the best match in UniProt
Protein Sequence (678 amino acids)
>Rv0050 Probable bifunctional penicillin-binding protein 1A/1B PonA1 (murein polymerase) (PBP1): penicillin-insensitive transglycosylase (peptidoglycan TGASE) + penicillin-sensitive transpeptidase (DD-transpeptidase) (Mycobacterium tuberculosis H37Rv) VVILLPMVTFTMAYLIVDVPKPGDIRTNQVSTILASDGSEIAKIVPPEGNRVDVNLSQVP MHVRQAVIAAEDRNFYSNPGFSFTGFARAVKNNLFGGDLQGGSTITQQYVKNALVGSAQH GWSGLMRKAKELVIATKMSGEWSKDDVLQAYLNIIYFGRGAYGISAASKAYFDKPVEQLT VAEGALLAALIRRPSTLDPAVDPEGAHARWNWVLDGMVETKALSPNDRAAQVFPETVPPD LARAENQTKGPNGLIERQVTRELLELFNIDEQTLNTQGLVVTTTIDPQAQRAAEKAVAKY LDGQDPDMRAAVVSIDPHNGAVRAYYGGDNANGFDFAQAGLQTGSSFKVFALVAALEQGI GLGYQVDSSPLTVDGIKITNVEGEGCGTCNIAEALKMSLNTSYYRLMLKLNGGPQAVADA AHQAGIASSFPGVAHTLSEDGKGGPPNNGIVLGQYQTRVIDMASAYATLAASGIYHPPHF VQKVVSANGQVLFDASTADNTGDQRIPKAVADNVTAAMEPIAGYSRGHNLAGGRDSAAKT GTTQFGDTTANKDAWMVGYTPSLSTAVWVGTVKGDEPLVTASGAAIYGSGLPSDIWKATM DGALKGTSNETFPKPTEVGGYAGVPPPPPPPEVPPSETVIQPTVEIAPGITIPIGPPTTI TLAPPPPAPPAATPTPPP