Protein Info for RS_RS25145 in Ralstonia solanacearum GMI1000

Annotation: hybrid sensor histidine kinase/response regulator

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 796 PF13426: PAS_9" amino acids 31 to 133 (103 residues), 19 bits, see alignment E=5.7e-07 amino acids 162 to 260 (99 residues), 29.5 bits, see alignment E=3e-10 amino acids 290 to 389 (100 residues), 14.6 bits, see alignment E=1.3e-05 PF08447: PAS_3" amino acids 42 to 127 (86 residues), 39.4 bits, see alignment E=2.4e-13 TIGR00229: PAS domain S-box protein" amino acids 143 to 267 (125 residues), 50.4 bits, see alignment E=1.2e-17 amino acids 274 to 396 (123 residues), 27.2 bits, see alignment E=1.8e-10 PF13188: PAS_8" amino acids 148 to 193 (46 residues), 16.2 bits, see alignment 3.3e-06 amino acids 277 to 320 (44 residues), 21.5 bits, see alignment 6.8e-08 PF00989: PAS" amino acids 151 to 258 (108 residues), 37.2 bits, see alignment E=1.1e-12 amino acids 274 to 321 (48 residues), 27.9 bits, see alignment 8.3e-10 PF08448: PAS_4" amino acids 153 to 263 (111 residues), 58.3 bits, see alignment E=3.3e-19 amino acids 279 to 391 (113 residues), 53.2 bits, see alignment E=1.3e-17 PF00512: HisKA" amino acids 417 to 482 (66 residues), 44.1 bits, see alignment E=6.9e-15 PF02518: HATPase_c" amino acids 530 to 642 (113 residues), 100.2 bits, see alignment E=3.8e-32 PF00072: Response_reg" amino acids 669 to 790 (122 residues), 68.9 bits, see alignment E=1.7e-22

Best Hits

KEGG orthology group: None (inferred from 100% identity to rso:RS02235)

Predicted SEED Role

"FIG00975311: hypothetical protein"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8XPG5 at UniProt or InterPro

Protein Sequence (796 amino acids)

>RS_RS25145 hybrid sensor histidine kinase/response regulator (Ralstonia solanacearum GMI1000)
MKEPVVSHFVPERGITSAPGRSYGTEPLDVFWVDDLLDGTRLHVSAAARQRWGMSPETLC
GPREHWLAHVHSGDHAALRAAWQGLAQGRDFALEYRWIGTDGAEHRVRECGYRLPAQGGA
ATHAVGVVEDMTQRLRTLAELTESEFQLQWLAESIPFPIVQLGPDHCIRFANHAYAQRFG
LPAADVLGRHVRELMGHEAYQRVLAHLMKGFAGQRVDFEMELEYPYVGPGRRFIHAAYTP
QRGPDGKVVALVCIIEDITERKEAERERFRHQREFVTLVENAPDIIARLDPALRCVYINR
AVTEAFGVPSGELIGQALSDSGLPACITGPLEAAAQCAFDTGGEQALTLHLEADAARPHV
AHYNARVIPEADRHGEMESALLIIYDVSERTEAERERNALLLREQAARAQAEAAAHARDQ
FLAVVSHELRSPLNGIQSWAYVLENQLAEGSQPVQRALAGIRTGVAQQVRLIEDLLDATR
VMSGKLRLTRQPFPLRTALERALDSVRAQAAERRITLHEAWSLGTEEIDGDADRIQQIVW
NLLSNAIKFTQEGGDVWLAADVSGEVARIVVRDNGRGIAPAFVPHLFDPFRQADGSHTRR
TGGLGLGLALVKRLTELHGGLVHAQSDGEQHGAIFTVYLPLHEGAELTAALAEDVVRPGP
LPSLGGLRVLLIDDQQDAREALATLLGQVHAEVGSAGSGDEAIAQLEAWGGLRRPHAVVC
DIALPDEDGYAILQRIRGWERRHLPAGVAQVPAIALTAFAQPHDRARALASGFQEHLSKP
VSPRDLVRALRTLARR