Protein Info for RS_RS24960 in Ralstonia solanacearum GMI1000

Annotation: NAD(P)-dependent oxidoreductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF02719: Polysacc_synt_2
  • amino acids 10 to 128 (119 residues), 25.1 bits, E=2.9e-09
• PF01370: Epimerase
  • amino acids 10 to 197 (188 residues), 75.5 bits, E=1.4e-24
• PF01073: 3Beta_HSD
  • amino acids 11 to 132 (122 residues), 38.2 bits, E=2.6e-13
• PF16363: GDP_Man_Dehyd
  • amino acids 11 to 135 (125 residues), 30.2 bits, E=1e-10
• PF13460: NAD_binding_10
  • amino acids 14 to 135 (122 residues), 35.1 bits, E=3.8e-12
• PF07993: NAD_binding_4
  • amino acids 47 to 197 (151 residues), 32 bits, E=2.1e-11

Best Hits

KEGG orthology group: None (inferred from 100% identity to rso:RS02194)

Predicted SEED Role

"UDP-glucose 4-epimerase (EC 5.1.3.2)" in subsystem Lacto-N-Biose I and Galacto-N-Biose Metabolic Pathway or Lactose and Galactose Uptake and Utilization or N-linked Glycosylation in Bacteria or Rhamnose containing glycans or linker unit-arabinogalactan synthesis (EC 5.1.3.2)

MetaCyc Pathways

• UDP-α-D-galactose biosynthesis (1/1 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis I (1/1 steps found)
• superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis (4/6 steps found)
• UDP-α-D-galactofuranose biosynthesis (1/2 steps found)
• colanic acid building blocks biosynthesis (7/11 steps found)
• UDP-N-acetyl-D-galactosamine biosynthesis II (4/7 steps found)
• D-galactose detoxification (1/3 steps found)
• D-galactose degradation I (Leloir pathway) (2/5 steps found)
• stachyose degradation (2/7 steps found)
• UDP-sugars interconversion (2/9 steps found)
• teichuronic acid biosynthesis (B. subtilis 168) (2/9 steps found)
• superpathway of mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (12/33 steps found)
• mycolyl-arabinogalactan-peptidoglycan complex biosynthesis (1/18 steps found)

KEGG Metabolic Maps

• Galactose metabolism
• Nucleotide sugars metabolism

Isozymes

Compare fitness of predicted isozymes for: 5.1.3.2

Use Curated BLAST to search for 5.1.3.2

Sequence Analysis Tools

See Q8XPK4 at UniProt or InterPro

Protein Sequence (305 amino acids)

>RS_RS24960 NAD(P)-dependent oxidoreductase (Ralstonia solanacearum GMI1000)
MAASQTPARILVTGVAGNLGRKVVEALASTPWCTSIIGVDWVEQSMQFSPQAAQRLRWVV
ADLTQADGAWTALLDEVDAVIHLAAIHSTPDATWEQALASYGMTLNVLQAAATRGVRRFV
FASSNHAMGAYKDQPLAGTIGPGRLIAELAPAPGTRWHNGIEEVYSLAYGTSKAMGERLC
KAVAAVSGGRLSVVALRIGWALPDDNDPNDINHSGTADTPVPGSVPDEASRIALRWFRNM
WLSNDDLRQLFIAAVTADPARWPAPAIVVNGVSNNTGMDWGLETGRALIGYEPQDDLYAR
LTQPA