Protein Info for RS_RS24370 in Ralstonia solanacearum GMI1000
Annotation: nitric-oxide reductase large subunit
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K04561, nitric oxide reductase, cytochrome b-containing subunit I [EC: 1.7.2.5] (inferred from 100% identity to rso:RSp1505)MetaCyc: 50% identical to nitric oxide reductase (menaquinol) (Cupriavidus necator H16)
RXN-12129 [EC: 1.7.5.2]
Predicted SEED Role
"Nitric-oxide reductase (EC 1.7.99.7), quinol-dependent" in subsystem Denitrification or Flavohaemoglobin or Nitrosative stress (EC 1.7.99.7)
MetaCyc Pathways
- nitrate reduction I (denitrification) (4/5 steps found)
- nitrate reduction VII (denitrification) (4/5 steps found)
- nitrifier denitrification (3/6 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 1.7.2.5 or 1.7.5.2 or 1.7.99.7
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q8XPY1 at UniProt or InterPro
Protein Sequence (756 amino acids)
>RS_RS24370 nitric-oxide reductase large subunit (Ralstonia solanacearum GMI1000) MNTNHRLWRWLGLIFVLSFGALGYLGRQIYLAAPPIPQAVVTTAGDVLFTGEQIQRGQQA WLAAGGQQLGTVWGHGSYVAPDWSADWLHREATALQAIHTQQQFGSAASLTAAQQAAVDV TVKEEMRRNTYDAARDSITVSKERARAIHGVAQHFEALFGTDPSLATLRDQYAMATGALP DQADREALAAFFFWTSWAAATDRPGETDVSYTSNWPHEPLVGNTMTSDAAMWSMVSIILL LAGIAAMLWLHGSGKHEEDAKPLPADPFLNVVATPSMKATRKYFFAVIGLILVQIGMGAI TAHYAVEGEAFFGIPLAQVLPYVISRTVHTQLGVLWIATAWLATGLYIAPLLSGREPKFQ KLGVDLLFWALIFIVVGSIATGWLGTLQHRGADFGFWIGNQGLEYTSMGRVWQLLLFVGL LFWVALLGRALWPALKTPSESRGLIAMVFLSATCIGGFYSTSLVWGQHTHYSMIEYWRWW LVHLWVEGFFEVFATAVVALLFTRLGLVRAASANRAIVAETIVFLFGGILGTLHHLYFTG TPTSIISVGAVFSALEVVPLTLIGLEALQTYRRMRGTPWLGAYKWPVLCFVAVGFWNTVG AGLLGFAINPPASLYYVQGLNMTAAHGHAALFGVYGMLGIGLMLFCLRGLYARSLHADRL LKPAYWGLNIGLAMMVFMSLLPAGIYQAWASITKGMWYARSPEVVHSKLMETLVWLRVPG DIVFAGGAFILALYALRLLRRPPSRQAATAPATATR