Protein Info for RS_RS23920 in Ralstonia solanacearum GMI1000

Annotation: chemotaxis protein CheA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 726 PF01627: Hpt" amino acids 8 to 105 (98 residues), 65.8 bits, see alignment E=8.5e-22 PF09078: CheY-binding" amino acids 176 to 237 (62 residues), 79.5 bits, see alignment 3.9e-26 PF02895: H-kinase_dim" amino acids 308 to 370 (63 residues), 70.8 bits, see alignment E=2.7e-23 PF02518: HATPase_c" amino acids 418 to 554 (137 residues), 64.8 bits, see alignment E=2.4e-21 PF01584: CheW" amino acids 560 to 689 (130 residues), 100 bits, see alignment E=2.3e-32

Best Hits

KEGG orthology group: K03407, two-component system, chemotaxis family, sensor kinase CheA [EC: 2.7.13.3] (inferred from 100% identity to rso:RSp1408)

Predicted SEED Role

"Signal transduction histidine kinase CheA (EC 2.7.3.-)" in subsystem Bacterial Chemotaxis or Flagellar motility or Two-component regulatory systems in Campylobacter (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3, 2.7.3.-

Use Curated BLAST to search for 2.7.13.3 or 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8XQ78 at UniProt or InterPro

Protein Sequence (726 amino acids)

>RS_RS23920 chemotaxis protein CheA (Ralstonia solanacearum GMI1000)
MNLDLSQFFGAFFEEAEELLVDMERLLLDLDVANPSSDDLNAIFRCAHSIKGGAATFGFT
HMTELTHVLESILDRARTGTLQLRENMVDAFLETKDVLKSQLEAYRHEHPIDTATLEYMV
AKLNSLTAEDGAPATAAAPAAAAPAAAPAAAAAEPQAPAPAATEPPIGAIDGGLDVKLVN
VSDEDCELIVTELKHLGTVLRHARSGRNSDIVLQSTCTPDDIIAVSCFIIDADQIVIAPH
VADPASAAVAPAAPAAVAEAAVAAPAPAAAQTTAHAPAANEAARPPASAAPAPSRPAAAE
KPAVAAETSIRVGVEKVDQLINLVGELVITQAMLAQTASAFDPVLNERLFSGLSQLTRNA
RDLQEAAMSIRMMPMDYVFNRFPRLVRDLAHKLGKQVELSTFGKSTELDKGLIERIIDPL
THLVRNSLDHGIEKPEARVANGKDATGQLLLSAAHQGGNIVIEVSDDGQGLNRDKILKKA
RERGLPVSDNMTDDEINQLIFAPGFSTADQVTDVSGRGVGMDVVKQNIQSMGGYVEIQSH
AGRGTTIRIVLPLTLAILDGMSVKTGDEVFILPLSCVAESLQPRPEDIKAVPGGGRLLKV
RNEYLTLVPLYERFRITPSIADPSEGIVVILDSEGKKIALQIDELVGQQQVVVKNLETNY
RKVSGISGATILGDGSVALIIDVSALMRETRAGHSESAVRAVSAEKPEMTSAPADGRFST
EAMAAA