Protein Info for RS_RS23325 in Ralstonia solanacearum GMI1000

Annotation: carbohydrate kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF00294: PfkB
  • amino acids 9 to 295 (287 residues), 142.2 bits, E=1.2e-45

Best Hits

KEGG orthology group: K00847, fructokinase [EC: 2.7.1.4] (inferred from 100% identity to rso:RS05325)

Predicted SEED Role

"Fructokinase (EC 2.7.1.4)" in subsystem Fructose utilization or Mannitol Utilization or Ribitol, Xylitol, Arabitol, Mannitol and Sorbitol utilization or Sucrose utilization or Sucrose utilization Shewanella (EC 2.7.1.4)

MetaCyc Pathways

• superpathway of anaerobic sucrose degradation (16/19 steps found)
• heterolactic fermentation (15/18 steps found)
• sucrose degradation III (sucrose invertase) (4/4 steps found)
• D-sorbitol degradation I (3/3 steps found)
• sucrose degradation II (sucrose synthase) (4/5 steps found)
• sucrose degradation IV (sucrose phosphorylase) (3/4 steps found)
• sucrose degradation I (sucrose phosphotransferase) (2/3 steps found)
• mannitol cycle (2/5 steps found)
• sucrose degradation VII (sucrose 3-dehydrogenase) (1/4 steps found)

KEGG Metabolic Maps

• Fructose and mannose metabolism
• Nucleotide sugars metabolism
• Starch and sucrose metabolism

Isozymes

Compare fitness of predicted isozymes for: 2.7.1.4

Use Curated BLAST to search for 2.7.1.4

Sequence Analysis Tools

See Q8XQE7 at UniProt or InterPro

Protein Sequence (307 amino acids)

>RS_RS23325 carbohydrate kinase (Ralstonia solanacearum GMI1000)
MLTTMNLPQLVVFGEALTDFIREADGRWRSVAGGSCWNVARVCARLGAPTGFAGTVSRDV
FGDELTALSRQAGLDMRFMRQVARPPLLAMVPAAHPPQYFFIGENSADLAFDTGVLPDGW
IEAAQIVHFGSLSLARQPLAAHLLETATAVHAAGKRIAFDPNYRDAMAGPDYRPTLRRMA
GLASYIKVSDEDLRGLFPELDETSALAQLRAWAPTAAILMTRGAAGMTLITPEGTLFQPA
LPTLVADTVGAGDASMGGWLASLLTRPEAMPASHLEFSAASAAAVCARQGAYAPTREEIV
SALAVSR