Protein Info for RS_RS22760 in Ralstonia solanacearum GMI1000
Annotation: choline dehydrogenase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
KEGG orthology group: K03333, cholesterol oxidase [EC: 1.1.3.6] (inferred from 100% identity to rso:RS05823)Predicted SEED Role
No annotation
MetaCyc Pathways
- cholesterol degradation to androstenedione I (cholesterol oxidase) (3/17 steps found)
- superpathway of cholesterol degradation I (cholesterol oxidase) (9/42 steps found)
KEGG Metabolic Maps
Isozymes
No predicted isozymesUse Curated BLAST to search for 1.1.3.6
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q8XQQ4 at UniProt or InterPro
Protein Sequence (1150 amino acids)
>RS_RS22760 choline dehydrogenase (Ralstonia solanacearum GMI1000) MVRIATPLSEIKEHYDIVVIGSGYGGGIAASRMARAGRTVCLLERGREIIPGEYPNTLAE ATEEFQVHHPDGHIGSRTGLYDLHVNAQQNVVVGCGLGGTSLINANVSLEPEPQVFEDPR WPRHVREHADTLLKEGFARAREMLRPTPYPGTAAALPKLQANEKSASQMQAPHTFYRPPI NVTFDDLPDNRNHVGVEQLRCNDCGDCVSGCNNKAKNTTLMNYLPDASNHGAEIFCQASV RYLERDGDGWIVHYQYVGTGREAFDAPTLFVKANIVVLSAGTLGSTEILLRSRTQGLLLS EKLGKHMSGNGDILGFGYNNNDKINGIGFGAHSGKGIGPVGPCITSIIDMRDEKDWTQRM VIEEGSIPGALGRAIVPAMAAASTQIGRPTSTGVAAKIAHAAREVESAVLGPYYGAVNRL QTYLIMSHDNGAGEMKLDDHDQLRVHWPGVGEQPNFKIGNDRLYDATRALGGTYVPNPIW TDLFKHSLITVHPLGGCVMGEDAAQGVVNHKGQVFSSASGSDVYPGLYVTDGSVIPTSLA VNPLLTISAVSERTMALLAQDRGWKIDYTLPSAPSKPARPVKPGIEFTEAMKGYVSLAHR QAQGTDPAVYEAAYQAGKDAGSTMAFIVTIRSDDLDQLIANPQHPATIVGTLNAPALSAR PLTVSHGVFNLFEAFPEQVGVRHMNYNMALTAEDGKEYFFSAFKSVPSDHSVLRAWADTS TLYVTVYDGTDNTGPVVGSGVLHIEPADFAKQMTTMKVLNASGEAERLKDLARFGKYFAG VLWESYGGVLAGNAYFNPDAPPRKKRPLNAGAPEVLFFQTEDNVTLRLTRFQGGSKGPVM LVHGLGVGSNIFSTDTIATNLLEFLYQHGYDVWLLDFRVSILLEASRQQCDGDQIARYDF PAAIDRIRQATGADDVQCVVHCYGATTFFMSLLAGLQGVRSVVCSQIATDIVVPPATAAK TGLHIPSVLDKLGVKSLTAYTQANADWLEKLYDTALKGYALVEAQGYCNNPVCHRITFMY ASLYRHETLNESLHDNLHELFGVANMRTMEHLALMCRTGHLVGFGGEDIYMPHLDRLQLP ILFISGAQNECYLPESTRRTYQQLVDRFGPERYSRLVIPGYGHIDCMFGKNAAMDVYPAI LAHLDKTASA