Protein Info for RS_RS21115 in Ralstonia solanacearum GMI1000

Annotation: NUDIX domain-containing protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01467: CTP_transf_like
  • amino acids 12 to 149 (138 residues), 43.1 bits, E=4.9e-15
• TIGR00125: cytidyltransferase-like domain
  • amino acids 12 to 67 (56 residues), 40 bits, E=1.7e-14
• PF00293: NUDIX
  • amino acids 208 to 328 (121 residues), 78.4 bits, E=5.4e-26

Best Hits

Swiss-Prot: 46% identical to NADM_SYNY3: Bifunctional NMN adenylyltransferase/Nudix hydrolase (slr0787) from Synechocystis sp. (strain PCC 6803 / Kazusa)

KEGG orthology group: K13522, bifunctional NMN adenylyltransferase/nudix hydrolase [EC: 2.7.7.1 3.6.1.-] (inferred from 100% identity to rso:RS05360)

MetaCyc: 46% identical to nicotinamide mononucleotide adenylyltransferase subunit (Synechocystis sp. PCC 6803)
Nicotinamide-nucleotide adenylyltransferase. [EC: 2.7.7.1]

Predicted SEED Role

"Nicotinamide-nucleotide adenylyltransferase, NadM family (EC 2.7.7.1) / ADP-ribose pyrophosphatase (EC 3.6.1.13)" in subsystem NAD and NADP cofactor biosynthesis global or Nudix proteins (nucleoside triphosphate hydrolases) or NAD regulation (EC 2.7.7.1, EC 3.6.1.13)

MetaCyc Pathways

• NAD biosynthesis from nicotinamide (2/2 steps found)
• superpathway of NAD biosynthesis in eukaryotes (10/14 steps found)
• NAD salvage pathway IV (from nicotinamide riboside) (1/2 steps found)

KEGG Metabolic Maps

• Folate biosynthesis
• Lipopolysaccharide biosynthesis
• Nicotinate and nicotinamide metabolism
• Purine metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.-, 3.6.1.13

Use Curated BLAST to search for 2.7.7.1 or 3.6.1.- or 3.6.1.13

Sequence Analysis Tools

See Q8XRJ6 at UniProt or InterPro

Protein Sequence (345 amino acids)

>RS_RS21115 NUDIX domain-containing protein (Ralstonia solanacearum GMI1000)
MSTTTKRFDALVFVGRFQPLHRAHVEVLRRALSLADTVCVLIGSTDKPRTIKDPFSFDER
RQMILSVLSEAERERVRIGAVQDSTYNDVDWLRWVQDAVAAELGGTADRRIGLIGHEKDA
SSYYLRMFPQWPLVEVDASEDVSSTEIREQYFAERSNSFVSWAVPAPVFAWLEAFRNQPA
FAQLKAEAEFIATYRKAWSAAPYPVTFVTVDAVVVHSGHLLLVRRRSEPGRGLWALPGGF
VGQDERLETACIRELREETGLKLPEPVLRGSVKDRQVFDHPQRSLRGRTITHAFLFHFPV
GELPRVKGGDDADKARWVPLNDFARMRDVMFEDHFDIAYHFLGKL