Protein Info for RS_RS20250 in Ralstonia solanacearum GMI1000

Annotation: two-component sensor histidine kinase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 466 signal peptide" amino acids 1 to 32 (32 residues), see Phobius details transmembrane" amino acids 164 to 183 (20 residues), see Phobius details PF21085: CusS" amino acids 3 to 157 (155 residues), 70.5 bits, see alignment E=3.7e-23 TIGR01386: heavy metal sensor kinase" amino acids 4 to 455 (452 residues), 454.1 bits, see alignment E=3e-140 PF00672: HAMP" amino acids 181 to 233 (53 residues), 43.7 bits, see alignment 5.6e-15 PF00512: HisKA" amino acids 239 to 303 (65 residues), 49.1 bits, see alignment E=9.8e-17 PF02518: HATPase_c" amino acids 347 to 456 (110 residues), 88.3 bits, see alignment E=9.3e-29

Best Hits

KEGG orthology group: K07644, two-component system, OmpR family, heavy metal sensor histidine kinase CusS [EC: 2.7.13.3] (inferred from 100% identity to rso:RSp0654)

Predicted SEED Role

"Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-)" in subsystem Potassium homeostasis (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3, 2.7.3.-

Use Curated BLAST to search for 2.7.13.3 or 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8XS27 at UniProt or InterPro

Protein Sequence (466 amino acids)

>RS_RS20250 two-component sensor histidine kinase (Ralstonia solanacearum GMI1000)
MRRRLSLTARLTALFSLCSAAVLLGLGVVIALAMEQHFAVEDFTALGEHVSVIGKIVESG
PAPQAEARIRDALQHRTGFVARILGPDRRALYASDGFDFRAADEALARARPGSDRLVWAQ
GGQQYRAMHTTVALRDGTAGRLDMLVAMNTDIHAHFLHTFRGTLAFYIALAALASGVFGW
WAARRGLAPLRTLASRARTVTADKLDERMPVDTVPAEVADLAVTLNAMLERLQHDFRRLS
DFSTDIAHEIRTPITNLLTQTEVVLSQPREGAKYRDVLTSNAEELQRLARMVSDMLYLAK
MEHSLTLPSAEDIHLADELRALFEFYDALAEDKAVRLELRGDGRVSGDRLMLRRALSNLL
SNAIRHAPPRGAVLVSIADTGHGVTVSVDNDGDEIPPQVLSRIFERFYRADKSRARPESD
STGLGLAITKAIVVAHGGSIAVSRVGGRTRFALHFARRPEAGAAPH