Protein Info for RS_RS18735 in Ralstonia solanacearum GMI1000

Annotation: aconitate hydratase AcnA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01341: aconitate hydratase 1
  • amino acids 18 to 864 (847 residues), 971.5 bits, E=1.6e-296
• PF00330: Aconitase
  • amino acids 72 to 542 (471 residues), 479.3 bits, E=1.5e-147
• PF00694: Aconitase_C
  • amino acids 668 to 795 (128 residues), 112.9 bits, E=1.4e-36

Best Hits

KEGG orthology group: K01681, aconitate hydratase 1 [EC: 4.2.1.3] (inferred from 100% identity to rso:RSp0330)

Predicted SEED Role

"Aconitate hydratase (EC 4.2.1.3)" in subsystem Propionate-CoA to Succinate Module or Serine-glyoxylate cycle or TCA Cycle (EC 4.2.1.3)

MetaCyc Pathways

• superpathway of glycolysis, pyruvate dehydrogenase, TCA, and glyoxylate bypass (23/26 steps found)
• superpathway of glyoxylate bypass and TCA (11/12 steps found)
• partial TCA cycle (obligate autotrophs) (8/8 steps found)
• TCA cycle I (prokaryotic) (9/10 steps found)
• glyoxylate cycle (6/6 steps found)
• TCA cycle V (2-oxoglutarate synthase) (8/9 steps found)
• superpathway of cytosolic glycolysis (plants), pyruvate dehydrogenase and TCA cycle (17/22 steps found)
• superpathway of glyoxylate cycle and fatty acid degradation (11/14 steps found)
• TCA cycle II (plants and fungi) (7/9 steps found)
• TCA cycle IV (2-oxoglutarate decarboxylase) (7/9 steps found)
• TCA cycle VI (Helicobacter) (7/9 steps found)
• TCA cycle VII (acetate-producers) (7/9 steps found)
• nitrogen remobilization from senescing leaves (6/8 steps found)
• ethene biosynthesis V (engineered) (18/25 steps found)
• TCA cycle III (animals) (7/10 steps found)
• mixed acid fermentation (11/16 steps found)
• reductive TCA cycle I (6/11 steps found)
• reductive TCA cycle II (6/12 steps found)
• methylaspartate cycle (11/19 steps found)

KEGG Metabolic Maps

• Biosynthesis of alkaloids derived from histidine and purine
• Biosynthesis of alkaloids derived from ornithine, lysine and nicotinic acid
• Biosynthesis of alkaloids derived from shikimate pathway
• Biosynthesis of alkaloids derived from terpenoid and polyketide
• Biosynthesis of phenylpropanoids
• Biosynthesis of plant hormones
• Biosynthesis of terpenoids and steroids
• Citrate cycle (TCA cycle)
• Glyoxylate and dicarboxylate metabolism
• Reductive carboxylate cycle (CO2 fixation)

Isozymes

Compare fitness of predicted isozymes for: 4.2.1.3

Use Curated BLAST to search for 4.2.1.3

Sequence Analysis Tools

See Q8XSY7 at UniProt or InterPro

Protein Sequence (877 amino acids)

>RS_RS18735 aconitate hydratase AcnA (Ralstonia solanacearum GMI1000)
MENTCTPPEASIGVSDATYRYVDLPAMFDSALTRLPVVLRLLLENVIRNTQGEERQRAVN
GVLRWLDTGTSEEEIAFQPNRVLMHDTTSTPALVDIAAMRDALAEAGADPAALNPVLPVD
VSVDHSLAVEAYAQPDAAARNLRIEMRRNAERYRFLRWASNTLRGVRIHPPGTGIMHTIN
LEQLATVATVQTIDGARWLVPDTLIGTDSHTPMINGIGVLGWGVGGLEAQTVMFGMPVMQ
RIPDVIGVRLDGALPAGSSATDLALTVTKALRDIGVSGEFVEFFGPGVATLTAGERSVVA
NMAPEYGATTGFFPVDAQTLAYLRATGRTEHAIAQVEALHRRAGLWFDPDNTPRYTRVIE
IDLSTVHLHVAGPRRPQDLLPYTETGRALSAVAARAQPGKTLPTHPVAIAAITSCTNTSD
PAQLVAAGLVARRARERGLRVPAWVKTSLAPGSPAAAEYLKRAGLIDDLAAVGFDIVGFG
CTTCIGNPGPLTAPIQQAIAHGETAPVAVLSGNRNFPGRVHPDLSLGFIMSPPLVIAFAL
AGDAALDLSRQPLQTTADGTPVYLRDLWPTREEIDALVQLGTSPTDYRRAFATATANPMW
AAIDAPTTARYPWDAHSTALRRPPFASLDAGSQLGEFDAYPLLVLGDDITTDHISPASAI
PKDSFVADFLAARGDDRNDLNVFASRRGNWEVMVRAAFYNKTLVNLLAPDAPVAHTVHAP
SGEVMPIHEAARRYHDEGACVVLLAGARYGTGSSRDWAAKGQRLLGIRAVIAVSFERIHR
SNLIGMGILPLRFSAGVNPQALAIRPGDTVHLSALPERIEPRCTVPVRIQRADGRVEMFE
ATAAVETQLETALLRRGGVIPSILGDTLAPPHSRASV