Protein Info for RS_RS18285 in Ralstonia solanacearum GMI1000

Annotation: malto-oligosyltrehalose synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 940 TIGR02401: malto-oligosyltrehalose synthase" amino acids 11 to 938 (928 residues), 939.2 bits, see alignment E=1.2e-286 PF00128: Alpha-amylase" amino acids 32 to 115 (84 residues), 59.3 bits, see alignment E=2.6e-20

Best Hits

KEGG orthology group: K06044, (1->4)-alpha-D-glucan 1-alpha-D-glucosylmutase [EC: 5.4.99.15] (inferred from 100% identity to rso:RS05188)

Predicted SEED Role

"Malto-oligosyltrehalose synthase (EC 5.4.99.15)" in subsystem Maltose and Maltodextrin Utilization or Trehalose Biosynthesis (EC 5.4.99.15)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 5.4.99.15

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8XT80 at UniProt or InterPro

Protein Sequence (940 amino acids)

>RS_RS18285 malto-oligosyltrehalose synthase (Ralstonia solanacearum GMI1000)
MTPPASPTAVPRATLRLQLHRAFTFDHARALLDDIAALGISHLYVSPITTAQPGSMHGYD
VVDPTRVNPELGGEDALGRLVAALHARGMGLIVDIVPNHMAVGGAHNAWWLDVLENGPAS
AWAHAFDIQWQPPQPALHGKVLAPFLGEPYDTALQGGRLTLHYDPGAARLAIAYHDHRFP
IALADYACVLRGAGPSGERDTDAALDAVAERFAALQSTRALHARRAHADAARTALRDFAA
TDAGRARIDRAVAALNAAPEQLHALMARQSWRLAHWRCANDEINWRRFFDIGSLAGLSVE
RADVFEATHALIFRLYRQGWIDGVRIDHVDGLADPAGYCRALRQRLAAEDAHRPADRRLG
RPWIVVEKILAADEPMRTGWDIDGTSGYDFMNQAGALLHDAAGEATLTQAWLDWTGRPAA
EAHFRVTALAARRRILHEHFATELDAATWALHAVAQQQRDAHDVTWHAIRRALAELVVHL
SVYRTYADAHGRDTQDTAIVRRAIHDAMPHLRRVDQPLLARLDAWLGGEPAGHDRLRQLA
LRRGQQLSSPVAAKAVEDTACYRYGRLLSRNEVGADPGAFTLDAAAFHQAMAARARLWPH
AMLATATHDHKRGEDVRARLAVLSERPAHWLAAALPWRAAHARWVRPLPEGPAPPPDAQW
MLYQTLVGAWPPGLDWRDADGVRAFAERIAQWQHKALREAKLRTDWLAPDLDYEQACHDF
VFTLLTGEAAPAFLPSLAAFVRTIAPAGAVNGLAQMLLRVTVPGVPDLYQGTDLWDTSLV
DPDNRRPVDFAVRHRSLRALQTHPEHSLAPLLAHWTDGRIKQAVLARALGVRAAMPEVFA
AGRYLPLALSGSGGAHALAFAREHAGRWVVAIVPLHAAALLGHAAVPVFPAGAWRDTTVC
LPAPLASIPLHSVFDGQTLCGARLALGQTLGALPVALLHG