Protein Info for RS_RS17225 in Ralstonia solanacearum GMI1000
Annotation: UTP--glucose-1-phosphate uridylyltransferase
These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.
Protein Families and Features
Best Hits
Swiss-Prot: 49% identical to GTAB_STAA8: UTP--glucose-1-phosphate uridylyltransferase (gtaB) from Staphylococcus aureus (strain NCTC 8325)
KEGG orthology group: K00963, UTP--glucose-1-phosphate uridylyltransferase [EC: 2.7.7.9] (inferred from 100% identity to rso:RSp0006)MetaCyc: 49% identical to UTP--glucose-1-phosphate uridylyltransferase (Staphylococcus aureus)
UTP-monosaccharide-1-phosphate uridylyltransferase. [EC: 2.7.7.64, 2.7.7.9]
Predicted SEED Role
"UTP--glucose-1-phosphate uridylyltransferase (EC 2.7.7.9)" (EC 2.7.7.9)
MetaCyc Pathways
- superpathway of anaerobic sucrose degradation (16/19 steps found)
- UDP-α-D-glucose biosynthesis (2/2 steps found)
- sucrose biosynthesis I (from photosynthesis) (7/9 steps found)
- sucrose degradation II (sucrose synthase) (4/5 steps found)
- superpathway of UDP-glucose-derived O-antigen building blocks biosynthesis (4/6 steps found)
- sucrose biosynthesis II (5/8 steps found)
- colanic acid building blocks biosynthesis (7/11 steps found)
- D-galactose detoxification (1/3 steps found)
- stachyose degradation (2/7 steps found)
- type I lipoteichoic acid biosynthesis (S. aureus) (5/17 steps found)
KEGG Metabolic Maps
- Galactose metabolism
- Nucleotide sugars metabolism
- Pentose and glucuronate interconversions
- Starch and sucrose metabolism
Isozymes
Compare fitness of predicted isozymes for: 2.7.7.9
Use Curated BLAST to search for 2.7.7.64 or 2.7.7.9
Sequence Analysis Tools
PaperBLAST (search for papers about homologs of this protein)
Search CDD (the Conserved Domains Database, which includes COG and superfam)
Predict protein localization: PSORTb (Gram-negative bacteria)
Predict transmembrane helices and signal peptides: Phobius
Check the current SEED with FIGfam search
Find homologs in fast.genomics or the ENIGMA genome browser
See Q8XTU8 at UniProt or InterPro
Protein Sequence (321 amino acids)
>RS_RS17225 UTP--glucose-1-phosphate uridylyltransferase (Ralstonia solanacearum GMI1000) MNEKITKAVFPVAGLGTRFLPATKASPKEMLPIVDKPLIQYAVEEAAAAGITEMIFITGR AKRAIEDHFDKAYELESELAAKNKHALLEMVQSIKPAGVECFYVRQPETLGLGHAVLCAQ KLVRDEPFAVILADDLLDHEPSVMQQMTELYDHYRCSIVGVETIAPQASRSYGVVAGREW DDRLIKLDGIVEKPAPEHAPSNLGVVGRYILTPRIFDHLRNLAPGTGGEIQLTDAIQRLM AEEQILAYRYRGQRFDCGSKFGYLQATVELALRHPEVGADFEAYLYARMGLPHELPAAVE APASTVTPVSKKVLPLEGVAA