Protein Info for RS_RS16960 in Ralstonia solanacearum GMI1000

Annotation: sensor histidine kinase KdpD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 937 transmembrane" amino acids 428 to 449 (22 residues), see Phobius details amino acids 453 to 472 (20 residues), see Phobius details amino acids 479 to 496 (18 residues), see Phobius details amino acids 507 to 529 (23 residues), see Phobius details PF02702: KdpD" amino acids 24 to 232 (209 residues), 320.4 bits, see alignment E=1.2e-99 PF13493: DUF4118" amino acids 433 to 539 (107 residues), 117.8 bits, see alignment E=4.8e-38 PF13492: GAF_3" amino acids 559 to 684 (126 residues), 42 bits, see alignment E=3e-14 PF00512: HisKA" amino acids 704 to 769 (66 residues), 65 bits, see alignment 1.3e-21 PF02518: HATPase_c" amino acids 814 to 925 (112 residues), 90.3 bits, see alignment E=2.9e-29

Best Hits

KEGG orthology group: K07646, two-component system, OmpR family, sensor histidine kinase KdpD [EC: 2.7.13.3] (inferred from 100% identity to rso:RSc3385)

Predicted SEED Role

"Osmosensitive K+ channel histidine kinase KdpD (EC 2.7.3.-)" in subsystem Potassium homeostasis (EC 2.7.3.-)

Isozymes

Compare fitness of predicted isozymes for: 2.7.13.3, 2.7.3.-

Use Curated BLAST to search for 2.7.13.3 or 2.7.3.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8XU09 at UniProt or InterPro

Protein Sequence (937 amino acids)

>RS_RS16960 sensor histidine kinase KdpD (Ralstonia solanacearum GMI1000)
MDETRPNPDTLLAELQSGEEKAARGKLRIYFGASAGVGKTYAMLSAAKVARAEGIDVVVG
LVETHGRAETAALVEGLEHLPPRQVEYRGRTLPEFDLDAALARKPALILVDELAHSNVAG
SRHPKRWQDIEDLLAAGIDVWTTVNVQHLDSLNEAVGSITGIRVWETVPDAVFDSAAEVI
LVDLPADELLRRLAEGKVYLPEQARHAARNFFRKGNLIALRELALRRTADRVDDDVQAYR
RARRIENVWRTRETIVACLDPQGDGEQVIRSAARLAAQLECDWHAVAVVMPHLRAADARA
ERLHALLKMAEDAGARIETLAGTNAVEAITGYVRRHNITKVVVGRPPARRWRSARAIVVA
LRLAIGLERRLPSGDFAEALARQCPEVDVIRAAADPARVQLPPRAASIGRVDVRDTAQRA
AAEAEGTWLRPAYLAACVYVGAATLLSSLVRPVFDLANIVMLFLAAVVAVAMRHGRGPAA
LASVLSVALFDFFFVPPRYSFAVSDVQYLLTFAVMLAVGLLVGQLTAGLREQAEAAVQRE
AAARALYEAARELSAALTLDQIVSIGGRFVNATFGGRCAFFFVGPDGRLGAAQVARPEGT
PDAPSGMPNLDRVLADWTCQHGQPAGTGTHTLPSGSVLYLPLKAPMAIRGVLAVEPETFQ
VLAQPDNRRQIDACATLIAIAIERVHYVEIAQDALLRIESERLRNSLLAAVSHDLRTPLT
GLAGMAETLLRTQPPLSPLQAEAAEAIGEQAQRMRAMVTNLLDMARLQNHDVKLRLEWQS
IEELVGAALKGTPLPTHAVVVDDLADLPLLRCDGPLMERVLSNLLENAGKFAPAGTEVRI
SAGVVHDAAHGDEMCVRVRDHGPGVPAGAERVIFEKFTRGDKESATGGVGLGLAVCEAIV
SAHGGRIWVETPADGGACFVVALPASDQPAMEDDIPA