Protein Info for RS_RS16765 in Ralstonia solanacearum GMI1000

Annotation: alpha/beta hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF12146: Hydrolase_4
  • amino acids 36 to 267 (232 residues), 239.4 bits, E=5.3e-75
• PF00561: Abhydrolase_1
  • amino acids 40 to 264 (225 residues), 65.4 bits, E=9.9e-22
• PF12697: Abhydrolase_6
  • amino acids 41 to 243 (203 residues), 64.9 bits, E=2.7e-21

Best Hits

KEGG orthology group: None (inferred from 100% identity to rso:RSc3346)

Predicted SEED Role

"Lysophospholipase (EC 3.1.1.5); Monoglyceride lipase (EC 3.1.1.23); putative" (EC 3.1.1.23, EC 3.1.1.5)

MetaCyc Pathways

• monoacylglycerol metabolism (yeast) (2/4 steps found)
• phospholipid remodeling (phosphatidylethanolamine, yeast) (2/4 steps found)
• triacylglycerol degradation (1/3 steps found)

KEGG Metabolic Maps

• Glycerolipid metabolism
• Glycerophospholipid metabolism

Isozymes

Compare fitness of predicted isozymes for: 3.1.1.5

Use Curated BLAST to search for 3.1.1.23 or 3.1.1.5

Sequence Analysis Tools

See Q8XU47 at UniProt or InterPro

Protein Sequence (286 amino acids)

>RS_RS16765 alpha/beta hydrolase (Ralstonia solanacearum GMI1000)
MTQAPATVDALETRQRMKDGTELFVRTWLPAPEAGEPRGTVILVHGMAEHSGRYPHVAQV
LCELGLRVRAFDLRGHGRSGGPRMALDAPDNYLTDLAEILDAVVAEWNEMPFVLGHSMGG
LIVARFATARVRPVRGVLLSSPALRLKLPPGANAVLGLLSALAPKLPVPNPVDPARLSHD
PSVGAAYRADPQVQKTISASVLAFMLNAITQAQQDAPRLEAPMLLLAGGADTIVDPSGSR
DFCAGAPEDLRTLAWFETAYHELFNEAEPMRGEAFGTMRAWLAGRI