Protein Info for RS_RS14985 in Ralstonia solanacearum GMI1000

Annotation: thiol:disulfide interchange protein DsbD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 608 signal peptide" amino acids 1 to 32 (32 residues), see Phobius details transmembrane" amino acids 184 to 213 (30 residues), see Phobius details amino acids 225 to 249 (25 residues), see Phobius details amino acids 260 to 283 (24 residues), see Phobius details amino acids 305 to 334 (30 residues), see Phobius details amino acids 341 to 364 (24 residues), see Phobius details amino acids 373 to 395 (23 residues), see Phobius details amino acids 401 to 422 (22 residues), see Phobius details amino acids 435 to 456 (22 residues), see Phobius details PF11412: DsbD_N" amino acids 33 to 148 (116 residues), 110.8 bits, see alignment E=1.3e-35 PF13386: DsbD_2" amino acids 192 to 378 (187 residues), 39.2 bits, see alignment E=2.2e-13 PF02683: DsbD" amino acids 192 to 369 (178 residues), 73.7 bits, see alignment E=6.1e-24 PF13899: Thioredoxin_7" amino acids 492 to 573 (82 residues), 59 bits, see alignment E=1.3e-19 PF13098: Thioredoxin_2" amino acids 501 to 594 (94 residues), 39.9 bits, see alignment E=1.4e-13

Best Hits

Swiss-Prot: 100% identical to DSBD_RALSO: Thiol:disulfide interchange protein DsbD (dsbD) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K04084, thiol:disulfide interchange protein DsbD [EC: 1.8.1.8] (inferred from 100% identity to rso:RSc2990)

Predicted SEED Role

"Cytochrome c-type biogenesis protein DsbD, protein-disulfide reductase (EC 1.8.1.8)" in subsystem Biogenesis of c-type cytochromes or Periplasmic disulfide interchange (EC 1.8.1.8)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.8.1.8

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8XV41 at UniProt or InterPro

Protein Sequence (608 amino acids)

>RS_RS14985 thiol:disulfide interchange protein DsbD (Ralstonia solanacearum GMI1000)
MTLSAFLPLRRLLQLGLLLVAMATLALPARAADDFLPPEQAFRFSAVQIDGQTVEVKFAI
ADGYYMYRERLAVAADPATVTFAPLELPPGKVKFDDTFNKDVETYRHALVFRVKAREAAS
PFSLIVTSQGCADQGVCYPPMKSRFRVEPAAASPAPPAAPLEAAGSSDALGGRIASTLGG
GNLGAIAVLFLGLGLLLTFTPCVLPMLPILSAIVVGEHATRMRAAAVSVAYVLGMAVVYT
AVGVAAGLAGQGLQAALQNAWVLGAFAALMVVLSLAMFGLYELQLPAAWRHRLTQASNRL
SGGQVAGAAVMGALSALIVSPCVTPALAGALAYIAQTGNAMVGGAALFSMSIGMGVPLVL
VGVGAGNLLPRAGYWLVVTKAIFGFILLGVALWIVQPVLPAWLAMVAWAVLLIAAAVFLR
TFDSLPADAGPLPRLGKVVGVVLALAGAVQLVGVAAGGRDPLQPLAGVMRASAGAQPDAR
GVVFQRVKSVSEVDEAVRAATATGRPVMLDFYADWCVSCKEMERLTFTDARVRAALADVV
LLQADVTADGADDRALLKRFSLFGPPATIFFDAQGNQAPVRVVGFERAETFLASLRRALG
TAQAKPST