Protein Info for RS_RS14245 in Ralstonia solanacearum GMI1000

Annotation: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• PF01225: Mur_ligase
  • amino acids 30 to 106 (77 residues), 41.3 bits, E=2.4e-14
• TIGR01143: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-alanine ligase
  • amino acids 35 to 460 (426 residues), 419.6 bits, E=7.3e-130
• PF08245: Mur_ligase_M
  • amino acids 116 to 308 (193 residues), 135 bits, E=5.1e-43
• PF02875: Mur_ligase_C
  • amino acids 328 to 406 (79 residues), 48.8 bits, E=1.1e-16

Best Hits

KEGG orthology group: K01929, UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate--D-alanyl-D-alanine ligase [EC: 6.3.2.10] (inferred from 100% identity to rso:RSc2848)

Predicted SEED Role

"UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate--D-alanyl-D-alanine ligase (EC 6.3.2.10)" in subsystem Methicillin resistance in Staphylococci or Peptidoglycan Biosynthesis (EC 6.3.2.10)

MetaCyc Pathways

• peptidoglycan biosynthesis I (meso-diaminopimelate containing) (11/12 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis I (meso-diaminopimelate containing) (8/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis II (lysine-containing) (7/8 steps found)
• UDP-N-acetylmuramoyl-pentapeptide biosynthesis III (meso-diaminopimelate containing) (6/8 steps found)
• peptidoglycan biosynthesis III (mycobacteria) (11/15 steps found)
• peptidoglycan biosynthesis II (staphylococci) (12/17 steps found)
• peptidoglycan biosynthesis IV (Enterococcus faecium) (12/17 steps found)
• peptidoglycan biosynthesis V (β-lactam resistance) (11/17 steps found)

KEGG Metabolic Maps

• Lysine biosynthesis
• Peptidoglycan biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.10

Sequence Analysis Tools

See Q8XVI3 at UniProt or InterPro

Protein Sequence (482 amino acids)

>RS_RS14245 UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase (Ralstonia solanacearum GMI1000)
MSDALPVMMHATDAAAWMAGAHLVGPDAAIRRVTTDSRAVLPGDLFVALIGERFDAHDFL
PDVVARGAAAVLVSRPPVGIPDLSGVAVLTVADTRIALGQLAAGWRRRFPIPVVAVTGSN
GKTTVKEMISAVFACAVGEAARLATAGNLNNDIGLPLTLFRLTAQHRLAVLELGMNHPGE
TEVLAAIAQPTVALINNAQREHQEFMVSVEAVAAEHAAVLAALPADGVAVFPRDAANGGE
YAPLWRAAAGTRRVLDFGIEGGAVSAAVTDTADGQRIDVHAPGLRFSFTLPLLGVHNARN
ALAATACALAAGIAPEVIAQALSAFAPVKGRLQRKPGHHGGLVIDDTYNANPDSVRAAID
ALATLPAPRWLVLGDMGEVGTQGPAFHREIGAYARERGIDAVLATGELARHMVEAFGGGA
RHFASAEALIEQGVPAIAPGATVLVKGSRFMRMERIVDALVLKDPAADSQGAPGTTHTQQ
TH