Protein Info for RS_RS14080 in Ralstonia solanacearum GMI1000

Annotation: proline--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR00409: proline--tRNA ligase
  • amino acids 1 to 568 (568 residues), 752.2 bits, E=2.3e-230
• PF00587: tRNA-synt_2b
  • amino acids 95 to 461 (367 residues), 122.4 bits, E=3.5e-39
• PF04073: tRNA_edit
  • amino acids 257 to 380 (124 residues), 105.2 bits, E=3.7e-34
• PF03129: HGTP_anticodon
  • amino acids 477 to 570 (94 residues), 70.4 bits, E=1.7e-23

Best Hits

Swiss-Prot: 100% identical to SYP_RALSO: Proline--tRNA ligase (proS) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K01881, prolyl-tRNA synthetase [EC: 6.1.1.15] (inferred from 100% identity to rso:RSc2816)

MetaCyc: 57% identical to proline--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Cysteine--tRNA ligase. [EC: 6.1.1.16]; Proline--tRNA ligase. [EC: 6.1.1.16, 6.1.1.15]; 6.1.1.15 [EC: 6.1.1.16, 6.1.1.15]; 3.1.1.M26 [EC: 6.1.1.16, 6.1.1.15, 3.1.1.M26]

Predicted SEED Role

"Prolyl-tRNA synthetase (EC 6.1.1.15), bacterial type" (EC 6.1.1.15)

MetaCyc Pathways

• tRNA charging (20/21 steps found)
• pyoluteorin biosynthesis (1/7 steps found)
• thiocoraline biosynthesis (1/7 steps found)
• ergotamine biosynthesis (4/12 steps found)
• anguibactin biosynthesis (1/8 steps found)
• (2S,3E)-2-amino-4-methoxy-but-3-enoate biosynthesis (2/10 steps found)
• clorobiocin biosynthesis (1/10 steps found)
• pyochelin biosynthesis (1/11 steps found)
• superpathway of ergotamine biosynthesis (5/18 steps found)
• sulfazecin biosynthesis (3/16 steps found)
• pyrrolomycin biosynthesis (1/14 steps found)
• phosalacine biosynthesis (2/25 steps found)
• phosphinothricin tripeptide biosynthesis (2/25 steps found)
• colibactin biosynthesis (6/38 steps found)

KEGG Metabolic Maps

• Aminoacyl-tRNA biosynthesis
• Cysteine metabolism

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.16

Use Curated BLAST to search for 3.1.1.M26 or 6.1.1.15 or 6.1.1.16

Sequence Analysis Tools

See Q8XVL4 at UniProt or InterPro

Protein Sequence (574 amino acids)

>RS_RS14080 proline--tRNA ligase (Ralstonia solanacearum GMI1000)
MKASQFFISTLKEAPADAEIVSHKLMMRAGMIKKLGAGLYTYMPVGLRVIRKVEQIVREE
MNASGAVEVLMPVVQPAELWQETGRWDKMGDEMMRVKDRHERDLVIQPTSEEVVTDIART
EIRSYKQMPVNFYQIQTKFRDERRPRFGIMRGREFTMKDAYSFDRDAEGLKVSYEKMYGA
YTRIFQRFGLEFRAVAADNGAIGGSGSHEFHVIADTGEDAIVYSPDSDYAANIEAAEALA
PAMPRGAATEALTKTHTPKRAKCEAVAEQLGIALQRTVKSIVLAKDVEGGEPQIWLLLLR
GDHELNEIKASKVPGLADFRFATEGEILRAFGTPPGYLGPIGTKQPVKVVADRTVAAMSD
FVVGANEEDFHYTGVNWGRDLPEAEVYDLRNVVEGDPSPDGKGTLAICRGIEVGHVFMLG
TRYSESMSATFLDENGKTQPMMMGCYGIGITRILGAAIEQNYDARGIIWPVSIAPFEVVI
CPVGYDRSEAVRAEADRLHAELAAAGIDVILDDRGERPGAMFADWELIGVPFRVVVGERG
LKDGKLELQGRRDEAAAAVAPADVLATLKSRLAQ