Protein Info for RS_RS13775 in Ralstonia solanacearum GMI1000

Annotation: short-chain dehydrogenase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

Swiss-Prot: 40% identical to 3BHD1_EGGLE: 3-beta-hydroxycholanate 3-dehydrogenase (NAD(+)) 1 (Elen_0198) from Eggerthella lenta (strain ATCC 25559 / DSM 2243 / JCM 9979 / NCTC 11813 / VPI 0255)

KEGG orthology group: K00540, [EC: 1.-.-.-] (inferred from 100% identity to rso:RSc2753)

MetaCyc: 40% identical to NAD-dependent bile acid 3beta-dehydrogenase (Eggerthella lenta DSM 2243)
RXN-11472 [EC: 1.1.1.391]; 1.1.1.391 [EC: 1.1.1.391]; 1.1.1.391 [EC: 1.1.1.391]; 1.1.1.391 [EC: 1.1.1.391]

Predicted SEED Role

"3-oxoacyl-[acyl-carrier protein] reductase (EC 1.1.1.100)" in subsystem Fatty Acid Biosynthesis FASII or mycolic acid synthesis (EC 1.1.1.100)

MetaCyc Pathways

superpathway of fatty acids biosynthesis (E. coli) (50/53 steps found)
palmitate biosynthesis III (28/29 steps found)
superpathway of fatty acid biosynthesis II (plant) (38/43 steps found)
palmitate biosynthesis II (type II fatty acid synthase) (29/31 steps found)
anteiso-branched-chain fatty acid biosynthesis (30/34 steps found)
even iso-branched-chain fatty acid biosynthesis (30/34 steps found)
odd iso-branched-chain fatty acid biosynthesis (30/34 steps found)
tetradecanoate biosynthesis (mitochondria) (23/25 steps found)
superpathway of unsaturated fatty acids biosynthesis (E. coli) (19/20 steps found)
octanoyl-[acyl-carrier protein] biosynthesis (mitochondria, yeast) (12/12 steps found)
oleate biosynthesis IV (anaerobic) (13/14 steps found)
palmitoleate biosynthesis I (from (5Z)-dodec-5-enoate) (9/9 steps found)
superpathway of fatty acid biosynthesis I (E. coli) (14/16 steps found)
biotin biosynthesis I (13/15 steps found)
cis-vaccenate biosynthesis (5/5 steps found)
fatty acid elongation -- saturated (5/5 steps found)
gondoate biosynthesis (anaerobic) (4/4 steps found)
8-amino-7-oxononanoate biosynthesis I (9/11 steps found)
(5Z)-dodecenoate biosynthesis I (5/6 steps found)
stearate biosynthesis II (bacteria and plants) (5/6 steps found)
8-amino-7-oxononanoate biosynthesis IV (4/5 steps found)
(5Z)-dodecenoate biosynthesis II (4/6 steps found)
stearate biosynthesis IV (4/6 steps found)
iso-bile acids biosynthesis I (2/4 steps found)
bile acids 3-O-epimerization (1/4 steps found)
petroselinate biosynthesis (2/6 steps found)
2-allylmalonyl-CoA biosynthesis (2/8 steps found)
streptorubin B biosynthesis (20/34 steps found)
bile acids epimerization (1/12 steps found)
mycolate biosynthesis (25/205 steps found)
superpathway of mycolate biosynthesis (26/239 steps found)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.-.-.-, 1.1.1.100

Use Curated BLAST to search for 1.-.-.- or 1.1.1.100 or 1.1.1.391

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8XVS6 at UniProt or InterPro

Protein Sequence (265 amino acids)

>RS_RS13775 short-chain dehydrogenase (Ralstonia solanacearum GMI1000)
MTAPPTSFPPRLAGKVALVTGATQGIGAATARLFARHGARVIVNALVRDAAAEAFAASIG
HDGNVLLVQADVRYRDQADAMVAAGVARFGGIDVLVNNAGINVFSDPLALSEADWARCLS
VDLEGAWHCARAVLPHMLARGAGSIVNIASVHGHKIIPGAFPYPVAKHGLIGLTRALGIE
YAARGIRVNSISPGLILTPIAEAGFAAAPDPEAERRRQADLLPCKRIGEPEEVAYTALFL
ASDEARFINAADILIDGARSQLYHE