Protein Info for RS_RS13620 in Ralstonia solanacearum GMI1000

Annotation: FMN-binding glutamate synthase family protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 532 signal peptide" amino acids 1 to 26 (26 residues), see Phobius details PF01645: Glu_synthase" amino acids 153 to 470 (318 residues), 277.4 bits, see alignment E=1e-86

Best Hits

KEGG orthology group: None (inferred from 100% identity to rso:RSc2722)

Predicted SEED Role

"Ferredoxin-dependent glutamate synthase (EC 1.4.7.1)" in subsystem Ammonia assimilation or Glutamine, Glutamate, Aspartate and Asparagine Biosynthesis (EC 1.4.7.1)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 1.4.7.1

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8XVV5 at UniProt or InterPro

Protein Sequence (532 amino acids)

>RS_RS13620 FMN-binding glutamate synthase family protein (Ralstonia solanacearum GMI1000)
MLPRRYWAFAGVVLLFVVTAGFALAGRLHWACAAIPGMLAMLGVRDLTQPRHSVLRNYPL
WGHLRFLLEFIRPEIRQYFVEDDTDERPFSRAQRSIVYQRAKGEVDSRPFGTELDVKIAG
YEWIGHSLAPTRIASSDFRVLVGEGRAKPYSMSVFNISAMSFGALSANAIRALNRGAKLG
NFIHDTGEGSISPYHREEGGDLIWEIGSGYFGCRDADGRFDPDRFAEQARSPQVKMIEVK
LSQGAKPGHGGVLPAAKVTAEIAATRGVPIGQDCISPATHSAFSTPLGLLQFVDRLRTLS
GGKPTGFKLCIGHPWEFFGIVKAMLASGILPDFIVVDGAEGGTGAAPLEFTDHVGTPLQE
GLLLVHNTLVGTNLRDKIKIGASGKIVTAFDVARTLAMGADWCNAARGFMFALGCIQAQK
CHTDRCPTGVATQDKSRQKALVVPDKAQRVHQYHAHTLHALLELTQAAGLRHPVDFRAHH
IVRRVSGNEVQLLSTLLKYLEPGDLLAGRYRYQLYERYWPMAQADRFDPVAA