Protein Info for RS_RS13285 in Ralstonia solanacearum GMI1000

Annotation: MprA protease, GlyGly-CTERM protein-sorting domain-containing form

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 543 signal peptide" amino acids 1 to 33 (33 residues), see Phobius details transmembrane" amino acids 514 to 535 (22 residues), see Phobius details PF00082: Peptidase_S8" amino acids 165 to 459 (295 residues), 124 bits, see alignment E=3.9e-40 TIGR03867: MprA protease C-terminal rhombosortase-interaction domain" amino acids 515 to 539 (25 residues), 32.3 bits, see alignment (E = 5.5e-12)

Best Hits

KEGG orthology group: K14645, serine protease [EC: 3.4.21.-] (inferred from 100% identity to rso:RSc2653)

Predicted SEED Role

"FIG00976385: hypothetical protein"

Isozymes

Compare fitness of predicted isozymes for: 3.4.21.-

Use Curated BLAST to search for 3.4.21.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8XW22 at UniProt or InterPro

Protein Sequence (543 amino acids)

>RS_RS13285 MprA protease, GlyGly-CTERM protein-sorting domain-containing form (Ralstonia solanacearum GMI1000)
MTSLFSRSKRGLRTGAGLLGAALMLASHAVPAAGTAQPQYTGDLIIKWRDDTSGTRKPLS
ATEASNRLLSVAQAAGNTLQVKRTLATDAQLMRTGLSDAAGIEAAAAKVAQDARVAYAVP
DRILYPTATPNDPLFSTQNNLQSPTVVAGGINVASAWDITLGSSSLVVAVVDTGYTDHPD
LSGKILPGYNFISDPARAGNSTGRGSDAHDTGDGVTSADVSAISGCTSSDIGNSTWHGTE
VMSVLAAGTNNALDIAGVGWNTRIVPVRTSGKCGALLSDTVDGMLWAGGISVSGVPANPN
PARVINVSLGSVGSCSAAEQDAINRLAALGTVVVAAAGNEGSAVDAPANCSGAIAVTAHV
DSGENASYANVGSQVALSAPGGGCANSQATSSGCTGPVSVIQADSNDGQYSLGNSVVKSV
AGTSFSTPEVAGTIALMLSVQSQLSNAQILAGLQQTARAHPSGTFCATRGGCGAGLLDTA
GAVRYAQTTTPAGIGSGSSSSSSSGSSSSSSGGGGGGGAIEMAGAALLLAAGLACRRRRT
TAR