Protein Info for RS_RS12000 in Ralstonia solanacearum GMI1000

Annotation: cobyric acid synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 495 PF13500: AAA_26" amino acids 13 to 239 (227 residues), 61.6 bits, see alignment E=1.4e-20 PF01656: CbiA" amino acids 14 to 239 (226 residues), 78.4 bits, see alignment E=7.4e-26 TIGR00313: cobyric acid synthase CobQ" amino acids 14 to 485 (472 residues), 470.6 bits, see alignment E=2.9e-145 PF07685: GATase_3" amino acids 260 to 444 (185 residues), 201 bits, see alignment E=2.3e-63

Best Hits

Swiss-Prot: 100% identical to COBQ_RALSO: Cobyric acid synthase (cobQ) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K02232, adenosylcobyric acid synthase [EC: 6.3.5.10] (inferred from 100% identity to rso:RSc2390)

MetaCyc: 52% identical to CobQ (Pseudomonas denitrificans (nom. rej.))
Adenosylcobyric acid synthase (glutamine-hydrolyzing). [EC: 6.3.5.10]

Predicted SEED Role

"Cobyric acid synthase (EC 6.3.5.10)" (EC 6.3.5.10)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.5.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8XWT0 at UniProt or InterPro

Protein Sequence (495 amino acids)

>RS_RS12000 cobyric acid synthase (Ralstonia solanacearum GMI1000)
MSTSPFSGPPRGTLMIQGTTSDAGKSTLVAGLCRIAHRAGVRVAPFKPQNMALNSAVTAD
GGEIGRAQALQAQAAGLAPTVDMNPVLLKPNSDTGAQVIIHGRPRGDLNARAYHDYKPTA
MAAVLASHGRLRAQYDLVLVEGAGSPAEVNLRARDIANMGFAEAVDCPVVLVADIDRGGV
FAHLVGTLACLSESERARVTGFVINRFRGDLSLLTPGLDWLTAQTGKPVFGVLPYLQGLH
LDAEDAVQTAQSAASGEVLRVVIPVLPRISNHTDFDALRAHPQVDVRMVGPGRPIPPADL
VILPGSKSVQADLAWLRAHGWDAAIARHLRYGGKLIGICGGMQMLGRRLRDPLGLEGRPG
SLDGLGYLDFETTLAPAKQLRQVRGTLADGGAALAGYEIHMGVTEGPALARPAVRLDDGR
TDGAVSADGQILATYLHGLFDAPEACRALLAWAGVREARAQDYAALREASLERLADTLRA
HLDLPALFASLAVRG