Protein Info for RS_RS11225 in Ralstonia solanacearum GMI1000

Annotation: valine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 800 850 900 968 transmembrane" amino acids 510 to 528 (19 residues), see Phobius details TIGR00422: valine--tRNA ligase" amino acids 9 to 945 (937 residues), 1056.4 bits, see alignment E=0 PF00133: tRNA-synt_1" amino acids 23 to 636 (614 residues), 463.9 bits, see alignment E=9.3e-143 PF09334: tRNA-synt_1g" amino acids 48 to 190 (143 residues), 26.7 bits, see alignment E=4.8e-10 PF08264: Anticodon_1" amino acids 687 to 840 (154 residues), 128.5 bits, see alignment E=4e-41 PF10458: Val_tRNA-synt_C" amino acids 899 to 963 (65 residues), 67.9 bits, see alignment 1.5e-22

Best Hits

Swiss-Prot: 80% identical to SYV_CUPNJ: Valine--tRNA ligase (valS) from Cupriavidus necator (strain JMP 134 / LMG 1197)

KEGG orthology group: K01873, valyl-tRNA synthetase [EC: 6.1.1.9] (inferred from 74% identity to bch:Bcen2424_1441)

Predicted SEED Role

"Valyl-tRNA synthetase (EC 6.1.1.9)" (EC 6.1.1.9)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8XX80 at UniProt or InterPro

Protein Sequence (968 amino acids)

>RS_RS11225 valine--tRNA ligase (Ralstonia solanacearum GMI1000)
MTDPNQSLAKSFEPAAIEQKWSAAWEAMGISRATLEAGRPDFCIQLPPPNVTGTLHMGHA
FNQTIMDGLARHARMQGANTLWVPGTDHAGIATQIVVERQLDAQGISRHDLGRPAFVDKV
WEWKEQSGSTITRQIRRMGASIDWEREYFTMDPKMSRAVSDVFVRLYEQGLIYRGKRLVN
WDPVLGTAVSDLEVVSEEEDGALWHIRYPLAQPDAKTGLTHLTVATTRPETMLGDVAVMV
HPEDERYAHLIGQAVRLPLSGEADALDTAQWREIPIIGDEYVDRAFGTGVVKVTPGHDFN
DYAVGQRHGLPQISVLTLEAKIADTAPATYRGIDRFEARKRIVVDLETLGLLAEVKKHTL
MVPRGDRTGVIIEPMLTDQWFVAMSKPAPEGTRFPGRSIAEVALDAVQGGKIKLVPEQWV
NTYNQWLNNIQDWCISRQLWWGHQIPAWYDEAGNVYVARTEEEARAQAAAKGHTGAIKRD
DDVLDTWFSAALVPFSSLGWPADTPELKHFLPSTVLVTGYDIIFFWVARMVMMTLHFTGE
VPFQTVYVHGLVRDSEGKKMSKSEGNTLDPVDLIDGIALEPLLVKRTTGLRRPKDAPNVE
KRTRKEFPDGIPAFGADALRFTFASLATLGRNINFDSSRCEGYRNFCNKLWNATRFVLMN
TEGQDCGLQECVGDCGPEGALHFSPADRWIVSLLQRVETEVEKGFADYRFDNIASAIYKF
VWDEYCDWYLELAKVQIQTGTPAQQRATRRTLLRVLETVLRLAHPIIPFITEELWQKVAP
MAGRAKGDGTESLAQQEYPRAVLAKIDEQAEQWVQQLKALVDACRNLRGEMSLSPAQRVP
LYAHGDAEFLQAAAPYVQALGKLSEVKVYTDAAAMEQDGAGAPVAIVGENKLLLKIEIDV
AAEHARLSKEIDRLRGEIVKCEGKLGNESFVARAPAAVVEQEQKRLADFKATLGKLEAQI
ARLPVQTA