Protein Info for RS_RS09945 in Ralstonia solanacearum GMI1000

Annotation: bifunctional tetrahydrofolate synthase/dihydrofolate synthase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01499: bifunctional protein FolC
  • amino acids 26 to 424 (399 residues), 337.7 bits, E=6.4e-105
• PF08245: Mur_ligase_M
  • amino acids 47 to 190 (144 residues), 35 bits, E=1.5e-12
• PF02875: Mur_ligase_C
  • amino acids 290 to 354 (65 residues), 28.7 bits, E=1.3e-10

Best Hits

KEGG orthology group: K11754, dihydrofolate synthase / folylpolyglutamate synthase [EC: 6.3.2.12 6.3.2.17] (inferred from 100% identity to rso:RSc1979)

Predicted SEED Role

No annotation

MetaCyc Pathways

• superpathway of tetrahydrofolate biosynthesis and salvage (11/12 steps found)
• superpathway of tetrahydrofolate biosynthesis (9/10 steps found)
• tetrahydrofolate biosynthesis I (3/3 steps found)
• superpathway of chorismate metabolism (43/59 steps found)
• folate polyglutamylation (4/5 steps found)
• tetrahydrofolate biosynthesis II (2/3 steps found)

KEGG Metabolic Maps

• Biosynthesis of phenylpropanoids
• Folate biosynthesis

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.2.12 or 6.3.2.17

Sequence Analysis Tools

See Q8XXY4 at UniProt or InterPro

Protein Sequence (432 amino acids)

>RS_RS09945 bifunctional tetrahydrofolate synthase/dihydrofolate synthase (Ralstonia solanacearum GMI1000)
MHCFDNLPDWLAHLETAHPVGIDMGLERISRVRDALALKLDPVVFTVGGTNGKGSTCAML
EAILMAAGYKVGCHTSPHLIDFNERARVNGEIATDAMLLPHFEAVERARCSFAEPVSLTY
FEFTTLAIMHLFAGAGLDAVILEVGLGGRLDAVNIIDPDCAVVTSVDLDHMAYLGDTREA
IGFEKAGIFRPGVPAVCSDPVPPVSLVRHAEAIGADLWLIGRDFNFQGDKQQWGWSGRGR
RWSSLGYPALRGANQLLNASAALAALDSVRDRLAITAQDVRVGLSRVALPGRFQVMPGRP
AVILDVAHNPHAAAALGQNLENMGFFRYTYAVFGAMQDKDIAGVLGHLLDKVDHWCLTDL
PTPRAASAAQLEQALADAGFSPGKEASVATYGDPATAYRNAMERATEDDRIVVFGSFFTV
AGVLAERKTRAH