Protein Info for RS_RS09930 in Ralstonia solanacearum GMI1000

Annotation: amidophosphoribosyltransferase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 511 TIGR01134: amidophosphoribosyltransferase" amino acids 2 to 464 (463 residues), 515.4 bits, see alignment E=8.5e-159 PF13522: GATase_6" amino acids 65 to 201 (137 residues), 59.1 bits, see alignment E=7.4e-20 PF13537: GATase_7" amino acids 81 to 213 (133 residues), 53.2 bits, see alignment E=4.4e-18 PF00156: Pribosyltran" amino acids 279 to 393 (115 residues), 37.7 bits, see alignment E=2e-13

Best Hits

Swiss-Prot: 59% identical to PUR1_PASMU: Amidophosphoribosyltransferase (purF) from Pasteurella multocida (strain Pm70)

KEGG orthology group: K00764, amidophosphoribosyltransferase [EC: 2.4.2.14] (inferred from 100% identity to rso:RSc1976)

MetaCyc: 60% identical to amidophosphoribosyltransferase (Escherichia coli K-12 substr. MG1655)
Amidophosphoribosyltransferase. [EC: 2.4.2.14]

Predicted SEED Role

"Amidophosphoribosyltransferase (EC 2.4.2.14)" in subsystem De Novo Purine Biosynthesis (EC 2.4.2.14)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 2.4.2.14

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8XXY7 at UniProt or InterPro

Protein Sequence (511 amino acids)

>RS_RS09930 amidophosphoribosyltransferase (Ralstonia solanacearum GMI1000)
MCGIVGVVSATPVNQLIYDSLLLLQHRGQDAAGIATAAGSTFHMHKANGMVRDVFRTRNM
RGLPGTIGIGQVRYPTAGSASEEEAQPFYVSAPFGIVLAHNGNLTNSEQLREEMFRRDRR
HINTHSDSEVLLNVLADELQRASNDIALSPEAIFKAVAGMHRRVRGSYAIVSQIAGYGML
AVRDPFGIRPLALGSQETPDGVEWMVASESVALEGIGFKFERDVAPGEAIFVDLEGQLHT
KQCADHPVLTPCIFEYVYLARPDSRMDGASVYDARLRMGDYLAEKIKREVTDPIDVVMPI
PDSSRPAAMQVANCLGVPYREGFFKNRYVGRTFIMPGQAVRKKSVRQKLNAMAIEFKGKN
VLIVDDSIVRGTTSSEIVQMARDAGAKKVIFASAAPPVKFPNVYGIDMPTRSELVAHGRT
HDEIARIIGADQLVYQDVEDMKRAVRDVNPALRDFEASCFDGHYVTGDIDEAYLDRLEAS
RNSAAACAARDSAQNDNPRTQLHLQPSAVNE