Protein Info for RS_RS09355 in Ralstonia solanacearum GMI1000

Annotation: MFS transporter

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 497 signal peptide" amino acids 1 to 19 (19 residues), see Phobius details transmembrane" amino acids 42 to 63 (22 residues), see Phobius details amino acids 71 to 92 (22 residues), see Phobius details amino acids 96 to 98 (3 residues), see Phobius details amino acids 100 to 120 (21 residues), see Phobius details amino acids 128 to 149 (22 residues), see Phobius details amino acids 158 to 179 (22 residues), see Phobius details amino acids 191 to 210 (20 residues), see Phobius details amino acids 218 to 241 (24 residues), see Phobius details amino acids 261 to 280 (20 residues), see Phobius details amino acids 300 to 319 (20 residues), see Phobius details amino acids 326 to 343 (18 residues), see Phobius details amino acids 350 to 374 (25 residues), see Phobius details amino acids 389 to 410 (22 residues), see Phobius details amino acids 465 to 483 (19 residues), see Phobius details TIGR00711: drug resistance MFS transporter, drug:H+ antiporter-2 (14 Spanner) (DHA2) family" amino acids 5 to 481 (477 residues), 441.7 bits, see alignment E=1.7e-136 PF07690: MFS_1" amino acids 9 to 402 (394 residues), 173.8 bits, see alignment E=2.5e-55

Best Hits

Swiss-Prot: 48% identical to EMRB_HAEIN: Multidrug export protein EmrB (emrB) from Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)

KEGG orthology group: K03446, MFS transporter, DHA2 family, multidrug resistance protein B (inferred from 100% identity to rso:RSc1852)

Predicted SEED Role

"Inner membrane component of tripartite multidrug resistance system" in subsystem Multidrug Resistance, Tripartite Systems Found in Gram Negative Bacteria

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8XYB0 at UniProt or InterPro

Protein Sequence (497 amino acids)

>RS_RS09355 MFS transporter (Ralstonia solanacearum GMI1000)
MLWLAAIVLAAANFVAVLDMTIANVSVPNISGNLGVSTSQGTWVITSYAVAEAILVPLTG
WLASRFGTVRVFAVAMGCFGLFSALCGLALSLGMLVAARICQGLAGGPLMPLSQTLLLRI
FPKEKAGAAIGLWSMTTIVAPVLGPILGGYLCDEYSWPWIFYINLPIAVGCAWAAWKMLA
RYESPIARNPIDKVGLALLVLWVGSLQIMLDEGKQLDWFASSTIAGLAVVAAIGFAAFLI
WELHAEHPAVNLRVFRHRGFSASVLTISLAFGAFFGANVLTPQWLQSFMGYTALEAGKTT
AWSGFFALFAAPIAAGLGGKVDARKLVFGGVIWLGIITLWRTVATTDMTYWQVAVPLMAM
GLGIPFFFVPTTGLAMASVDEEEMASAAGLMNFLRTLSGAFATSLITTLWDDRATLHHAE
LVGVSDGDGSVRTMLEGAGSTLDAINQSIDQLLTSQSVMLATNDIMAWVGVAFVLAASAI
WLAPKPRRTVAPGTGGH