Protein Info for RS_RS09170 in Ralstonia solanacearum GMI1000

Annotation: methionine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 496 PF09334: tRNA-synt_1g" amino acids 13 to 380 (368 residues), 137.9 bits, see alignment E=2e-44

Best Hits

KEGG orthology group: K01874, methionyl-tRNA synthetase [EC: 6.1.1.10] (inferred from 100% identity to rso:RSc1818)

Predicted SEED Role

"Methionyl-tRNA synthetase-related protein"

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 6.1.1.10

Use Curated BLAST to search for 6.1.1.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8XYE0 at UniProt or InterPro

Protein Sequence (496 amino acids)

>RS_RS09170 methionine--tRNA ligase (Ralstonia solanacearum GMI1000)
MKARKTYVLIPVMPTPNGPLHLGHIAGPFLKMDMLARHLRRNGNTVALVSATDPYETHVL
PRADEQNKPVEQICAENHRAIHRCLQALDIRYDAFIDPLASPYRARLNGITREVLDDLHA
QGRLHARNEPVHISRRTGRMLVGSRIVGTCPCCGVEMGGYHCEGCGMEVSPRDLIAPRAE
PADDTVEVEARASVFVDADLPALQRRMLEARVPADVRRIAERFMHAAGSAVRLSNPGEWG
EIWPNTLATAPSVVFSYTALFMLSVLCGEAAREILALDHNPFDRRSDALIVTSFGFDNTV
PFCVGVETLAQHSRRYRGFDRCLTNFFYTLDGRKFSTSRQHCIWADQAVRELGVASDVLR
YFLAKTSPESGPSDFSRDGFDAFRRAIEPRLAQMKAAVESGIGGDAAVPYADTLARLVAD
MDAAFDVDHFSLRAATRCIDQWLALPWQPAHAAAYVRGFCMLAYPVMPELASTLWSQLGH
EGLPRYLAATELEEAA