Protein Info for RS_RS08000 in Ralstonia solanacearum GMI1000

Annotation: amino acid permease

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 476 transmembrane" amino acids 31 to 52 (22 residues), see Phobius details amino acids 59 to 81 (23 residues), see Phobius details amino acids 91 to 112 (22 residues), see Phobius details amino acids 128 to 151 (24 residues), see Phobius details amino acids 157 to 176 (20 residues), see Phobius details amino acids 186 to 205 (20 residues), see Phobius details amino acids 218 to 242 (25 residues), see Phobius details amino acids 255 to 281 (27 residues), see Phobius details amino acids 302 to 325 (24 residues), see Phobius details amino acids 351 to 373 (23 residues), see Phobius details amino acids 379 to 398 (20 residues), see Phobius details amino acids 410 to 429 (20 residues), see Phobius details amino acids 435 to 454 (20 residues), see Phobius details PF13520: AA_permease_2" amino acids 26 to 431 (406 residues), 211.4 bits, see alignment E=3.7e-66 PF00324: AA_permease" amino acids 31 to 431 (401 residues), 137.4 bits, see alignment E=9.1e-44 PF13906: AA_permease_C" amino acids 409 to 458 (50 residues), 67 bits, see alignment 1.9e-22

Best Hits

Swiss-Prot: 65% identical to YHDG_BACSU: Uncharacterized amino acid permease YhdG (yhdG) from Bacillus subtilis (strain 168)

KEGG orthology group: K03294, basic amino acid/polyamine antiporter, APA family (inferred from 100% identity to rso:RSc1588)

Predicted SEED Role

"Amino acid transporters"

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8XZ18 at UniProt or InterPro

Protein Sequence (476 amino acids)

>RS_RS08000 amino acid permease (Ralstonia solanacearum GMI1000)
MSLFRTKNLDHMIRTGQGDTGLKKVFGPLDLTFLGVGAIIGTGIFVLTGTGALTAGPALT
LSFVVAALACGFAALCYAEFASTIPVSGSIYTYAYATLGELVAWIIGWDLMLEYGLATSA
VSVGWSGYFQSLMAGFGLHLPAALMAAPGAIPGVQTLFNLPALLIMLIITAVLSLGVRES
ARFNNIMVAIKVAVVVLFIVVGARHVQPANWQPFMPFGMSGVFGAAALVFFAFIGFDAVT
AAAEEVRNPERDLPIGIIGSLGVCTILYVVVAAIMTGIVPYTKFAGVDHPVSLALQMGGE
TWVAGFVDLGAIIGMTTVILVMGYGQTRVIFAMSRDGLLPKRLSRVHPRYATPFFNTWLV
GIVFALIAAFVPLNVLAELINIGTLAAFSLIAIAVLVLRRKRPDLPRGFRCPGVPFVPAA
AVVLCVFLMSQLQALTWIAFVVWMVIGLLVYFGYARSRSLLHAPDRAAVPGSEAAH