Protein Info for RS_RS07940 in Ralstonia solanacearum GMI1000

Annotation: threonine--tRNA ligase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 635 PF02824: TGS" amino acids 2 to 61 (60 residues), 40.5 bits, see alignment 4.6e-14 TIGR00418: threonine--tRNA ligase" amino acids 72 to 630 (559 residues), 733.7 bits, see alignment E=9.4e-225 PF07973: tRNA_SAD" amino acids 171 to 218 (48 residues), 59.6 bits, see alignment 4.8e-20 PF00587: tRNA-synt_2b" amino acids 319 to 528 (210 residues), 172.5 bits, see alignment E=1.9e-54 PF03129: HGTP_anticodon" amino acids 540 to 629 (90 residues), 76 bits, see alignment E=4.2e-25

Best Hits

Swiss-Prot: 100% identical to SYT_RALSO: Threonine--tRNA ligase (thrS) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K01868, threonyl-tRNA synthetase [EC: 6.1.1.3] (inferred from 100% identity to rso:RSc1577)

MetaCyc: 58% identical to threonine--tRNA ligase (Escherichia coli K-12 substr. MG1655)
Threonine--tRNA ligase. [EC: 6.1.1.3]; 6.1.1.3 [EC: 6.1.1.3]; 3.1.1.- [EC: 6.1.1.3]

Predicted SEED Role

"Threonyl-tRNA synthetase (EC 6.1.1.3)" (EC 6.1.1.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.1.1.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8XZ29 at UniProt or InterPro

Protein Sequence (635 amino acids)

>RS_RS07940 threonine--tRNA ligase (Ralstonia solanacearum GMI1000)
MIAITLPDGSRREFPGPVTVAEVAQSIGAGLAKAALAGRIDGQLVDTSFRIEQNVDLAIV
TDKDTDGLDVIRHSTAHLLAYAVKELYPEAQVTIGPVIENGFYYDFAYKRPFTPEDLVAI
EKRMAELAKKDEKVTREVWSRDDAVKLFEGMGEKYKAEIIASIPEDQEIGLYREGNFVDL
CRGPHVPSTGKLKVFKLMKVAGAYWRGDSNNEMLQRIYGTAWAKKEDQEAYLHMLEEAEK
RDHRKLGKLLDLFHLQEEAPGMVFWHPKGWQIWQAVEQYMRGRLAGAGYSEVRTPQVMDR
GLWERSGHWENYKENMFVTESEKREYAIKPMNCPGHVQIFNHGLRSYRDLPLRLAEFGAC
HRNEPSGALHGLMRVRGFVQDDAHIFCTEGQIMAEAKDFNDLAFSIYEDFGFENVAVKLA
LRPDKRAGTDEIWDHAEEGLRTALRACGVEWEELPGEGAFYGPKVEYHIKDAIGRSWQCG
TLQLDLVLPERLGAEYVAEDNSRKRPVMLHRAILGSFERFLGILLENHAGALPLWLAPEQ
VVIMSIADAHAEYAESVAQSLQKQGFRASADLRNEKITYKIREHSLQKVPYLVVVGDKER
DGNQVAVRARGNVDLGSMPVSTFVERLQNDFANKS