Protein Info for RS_RS07440 in Ralstonia solanacearum GMI1000

Annotation: ATP-dependent dethiobiotin synthetase BioD

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 229 transmembrane" amino acids 136 to 152 (17 residues), see Phobius details TIGR00347: dethiobiotin synthase" amino acids 8 to 178 (171 residues), 146.1 bits, see alignment E=5.5e-47 PF01656: CbiA" amino acids 8 to 210 (203 residues), 40.2 bits, see alignment E=3.2e-14 PF13500: AAA_26" amino acids 8 to 211 (204 residues), 134.5 bits, see alignment E=4.2e-43

Best Hits

Swiss-Prot: 100% identical to BIOD_RALSO: ATP-dependent dethiobiotin synthetase BioD (bioD) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K01935, dethiobiotin synthetase [EC: 6.3.3.3] (inferred from 100% identity to rso:RSc1479)

Predicted SEED Role

"Dethiobiotin synthetase (EC 6.3.3.3)" in subsystem Biotin biosynthesis (EC 6.3.3.3)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.3.3.3

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8XZC2 at UniProt or InterPro

Protein Sequence (229 amino acids)

>RS_RS07440 ATP-dependent dethiobiotin synthetase BioD (Ralstonia solanacearum GMI1000)
MPVRFDCFVTGTDTEIGKTLASAALLTALAGAGYRTAGLKPVAAGTLAGAPERTNEDIEQ
LRAAATVGLPMATLCPWLLDAPMSPHLAARRAGVTITLPPIVDALAQARTQADAVVVEGV
GGFRVPLSDTFDTAQLAVALGLPVVLVVGLRLGCLNHAALTAEAIAARGLRLAGWIGNVV
DATMAGLDDNVATLRRWIDAPHLGTIPRLPRPDARLAASHVDIAPLLAR