Protein Info for RS_RS07430 in Ralstonia solanacearum GMI1000

Annotation: adenosylmethionine--8-amino-7-oxononanoate transaminase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 468 TIGR00508: adenosylmethionine-8-amino-7-oxononanoate transaminase" amino acids 28 to 448 (421 residues), 502 bits, see alignment E=5.3e-155 PF00202: Aminotran_3" amino acids 41 to 445 (405 residues), 316.9 bits, see alignment E=8.8e-99

Best Hits

KEGG orthology group: K00833, adenosylmethionine-8-amino-7-oxononanoate aminotransferase [EC: 2.6.1.62] (inferred from 100% identity to rso:RSc1477)

Predicted SEED Role

"Adenosylmethionine-8-amino-7-oxononanoate aminotransferase (EC 2.6.1.62)" in subsystem Biotin biosynthesis (EC 2.6.1.62)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 2.6.1.62

Use Curated BLAST to search for 2.6.1.62

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8XZC4 at UniProt or InterPro

Protein Sequence (468 amino acids)

>RS_RS07430 adenosylmethionine--8-amino-7-oxononanoate transaminase (Ralstonia solanacearum GMI1000)
MAYPPIPVPADAAPSANTAWQARSRAAVWHPCTQNARLDAMPPLPIARGQGPWLIDFDGK
RYLDGTSSWWVNLFGHANPHINAALKAQLDMLEHTMLAGATHRPAVELAERLVGLTGGVL
GHSFFGSDGASAVEIALKMSFHAHRNAGQGARSRFVCLEHGYHGETLGALGVTDVAIFRD
AYDPLILQSHRVASPDARLAGPGETGADVAERALAALRDCLSANAGTIAAVIIEPLVQCA
AGMAMHDASYLRGVRALCDQFGVHWIADEIAVGCGRTGTFFACEQAGVWPDLLCLSKGIS
GGYLPLSIVLSRDAIYDAFNEDAPVRSFLHSHSYTGNPLACRAALATLDLFAEEDVFARN
RVTAARIAQGLAPLSHDARFAHLRQAGMITAFDVHPEVAGPRFAERFALTARAHGLLLRP
IGHTVYLLPPYVLTDDETDTLVERTLHTLEDTLAQATGAHEGETHAIA