Protein Info for RS_RS07015 in Ralstonia solanacearum GMI1000

Annotation: DNA ligase (NAD(+)) LigA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 813 PF01653: DNA_ligase_aden" amino acids 15 to 335 (321 residues), 371.9 bits, see alignment E=9.4e-115 TIGR00575: DNA ligase, NAD-dependent" amino acids 20 to 633 (614 residues), 659.3 bits, see alignment E=3.1e-202 PF03120: DNA_ligase_OB" amino acids 339 to 414 (76 residues), 114.1 bits, see alignment E=8.1e-37 PF03119: DNA_ligase_ZBD" amino acids 440 to 466 (27 residues), 43.9 bits, see alignment (E = 6.2e-15) PF12826: HHH_2" amino acids 559 to 621 (63 residues), 60.9 bits, see alignment 3.7e-20 PF00533: BRCT" amino acids 732 to 804 (73 residues), 53.6 bits, see alignment E=7.7e-18 PF12738: PTCB-BRCT" amino acids 746 to 795 (50 residues), 23.7 bits, see alignment 1.4e-08

Best Hits

Swiss-Prot: 100% identical to DNLJ_RALSO: DNA ligase (ligA) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K01972, DNA ligase (NAD+) [EC: 6.5.1.2] (inferred from 100% identity to rso:RSc1398)

Predicted SEED Role

"DNA ligase (EC 6.5.1.2)" in subsystem DNA Repair Base Excision (EC 6.5.1.2)

MetaCyc Pathways

Isozymes

No predicted isozymes

Use Curated BLAST to search for 6.5.1.2

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8XZJ7 at UniProt or InterPro

Protein Sequence (813 amino acids)

>RS_RS07015 DNA ligase (NAD(+)) LigA (Ralstonia solanacearum GMI1000)
MSKTEHPASGASPETRAAALRATLNRYAHEYYVLDQPSVPDAEYDRLYRELEALEAEHPE
LRTPDSPTLRVGGAVLPEFAPVRHVVPMLSIRTETDTTAGGALDFDASVRRELGLAESDP
PVEYAAELKFDGLAINLRYEKGFLVQAATRGDGATGEDVTQNIRTIRQIPLGLRPVGGAV
PDVLEVRGEVYMRRDDFERLNARQRERGDKTFVNPRNTAAGAVRQLDPKMAAERPLSFFA
YGLGEAAGWSGMPDTHSGMLDALVAYGFPVSKERAAVKGGEGLVQFHAAIGAKRDSLPFD
IDGVVYKVNSLALQRELGFRTREPRWAVAHKYPAQEALTTVESIGVQVGRTGAITPVARL
VPVFVGGVTVTNATLHNEDEVRRKDVRVGDTVIVRRAGDVIPEVVAVVLERRPMEDVPGS
DLFNPTQQPKHPPFELPRSCPVCGSHVVREEGEAVARCSGGLFCSAQRKEAIRHFAGRRM
MDIEGLGERYIDNLVELEYVHGIADLYRLTLDDFLEMKRRADERDGVTPETVAAGKIATK
WAENLLDGIRASKTPPLARFLFAMGIRHVGESTAKTLADWLGSLAIVRRAPAPLLLTLPD
VGATVAEAIADFFAEPKNQQALDALLTAGVAPQGEHPPSAKLRDQLEPAELYAALGVPKL
TAIRSKQLATLVPSLAQLANVDTAQLEGLPADVAASLLAWLDADDHRTRLGTLDALRAEL
LAAMPAGAAEEGALSGKTVVLTGTLPTLSRDEAKAMLEAAGAKVSGSVSKKTDYVVAGVE
AGSKLARAQELGVRVLDEAGMLALLQNPPGDSA