Protein Info for RS_RS07000 in Ralstonia solanacearum GMI1000

Annotation: alpha/beta hydrolase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 355 signal peptide" amino acids 1 to 33 (33 residues), see Phobius details PF12146: Hydrolase_4" amino acids 80 to 190 (111 residues), 58.6 bits, see alignment E=1.5e-19 PF00561: Abhydrolase_1" amino acids 82 to 330 (249 residues), 94.2 bits, see alignment E=2.6e-30 PF12697: Abhydrolase_6" amino acids 82 to 333 (252 residues), 79.4 bits, see alignment E=1.7e-25 PF06342: DUF1057" amino acids 105 to 187 (83 residues), 23 bits, see alignment E=9.4e-09 PF03096: Ndr" amino acids 127 to 181 (55 residues), 24.2 bits, see alignment 3.3e-09

Best Hits

KEGG orthology group: None (inferred from 100% identity to rso:RSc1396)

Predicted SEED Role

"2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate hydrolase (EC 3.7.1.-)" in subsystem Biphenyl Degradation or Central meta-cleavage pathway of aromatic compound degradation or carbazol degradation cluster (EC 3.7.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.7.1.-

Use Curated BLAST to search for 3.7.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8XZJ9 at UniProt or InterPro

Protein Sequence (355 amino acids)

>RS_RS07000 alpha/beta hydrolase (Ralstonia solanacearum GMI1000)
MLSRWMFAAVTVVFAAALSALSSSSPAAPAAAATDAATYGPRLEGFDYPAPVKLYPFASQ
GNALEMAYLDIEPRQPNGEVAVLLHGKNFCAATWAGTIAALAGAGYRVIAPDQIGFCKSS
KPRAYQYSFQQLASNTHALLVSLGIGQAIVIGHSTGGMLATRYALMYPAEVSRLVMINPI
GLEDWKAKGVPPMTVDQWYTREKQTTAERIRAYEQSTYYAGQWRADYEPWVQMLAGMYRG
PGRDLVAWSSALLYDMIYTQPVVYEFGQLRPWTLLLIGQKDTTAIAKDTAPPAVRSQLGN
YPELGRAAARAIPHATLVEFPDAGHAPQIQVPVALHNALLEWLASPEPARTRRPD