Protein Info for RS_RS06970 in Ralstonia solanacearum GMI1000

Annotation: succinyl-diaminopimelate desuccinylase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

• TIGR01246: succinyl-diaminopimelate desuccinylase
  • amino acids 6 to 381 (376 residues), 519.1 bits, E=3.1e-160
• PF01546: Peptidase_M20
  • amino acids 71 to 379 (309 residues), 153.6 bits, E=7.2e-49
• PF07687: M20_dimer
  • amino acids 183 to 290 (108 residues), 101.2 bits, E=3.1e-33

Best Hits

Swiss-Prot: 100% identical to DAPE_RALSO: Succinyl-diaminopimelate desuccinylase (dapE) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K01439, succinyl-diaminopimelate desuccinylase [EC: 3.5.1.18] (inferred from 100% identity to rso:RSc1390)

MetaCyc: 57% identical to succinyl-diaminopimelate desuccinylase (Escherichia coli K-12 substr. MG1655)
Succinyl-diaminopimelate desuccinylase. [EC: 3.5.1.18]

Predicted SEED Role

"N-succinyl-L,L-diaminopimelate desuccinylase (EC 3.5.1.18)" in subsystem Arginine Biosynthesis extended or Lysine Biosynthesis DAP Pathway (EC 3.5.1.18)

MetaCyc Pathways

• aspartate superpathway (23/25 steps found)
• superpathway of L-lysine, L-threonine and L-methionine biosynthesis I (16/18 steps found)
• L-lysine biosynthesis I (9/9 steps found)

KEGG Metabolic Maps

• Lysine biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 3.5.1.18

Use Curated BLAST to search for 3.5.1.18

Sequence Analysis Tools

See Q8XZK5 at UniProt or InterPro

Protein Sequence (383 amino acids)

>RS_RS06970 succinyl-diaminopimelate desuccinylase (Ralstonia solanacearum GMI1000)
MSPTLALTEDLIRRRSVTPEDKGCQDVLIERLSAAGFQCETVVSGPDHFRVTNLWAVKRG
RAGTDGKLLVFAGHTDVVPTGPVEQWRSDPFEPTHRDGKLYGRGAADMKTSIAGFVVAAE
EFVAVHPDHAGSIGFLITSDEEGPAHDGTIKVCDLLRTRGERLDYCVVGEPTSVSTLGDM
VKNGRRGSLSGKLTVNGVQGHIAYPHLAKNPIHLAVPALTALAAEQWDDGNAYFPPTTWQ
MSNIHGGTGATNVIPGHVIIDFNFRFSTASTPEGLKSRVHAILDAHGLDYTLAWTLGGEP
FLTERGELSEALSSAIGAECGVATELSTTGGTSDGRFIAKLCPQVIEFGPPNASIHKIDE
HVDVAFIEPLKNVYRRVLETLIA