Protein Info for RS_RS06920 in Ralstonia solanacearum GMI1000

Annotation: glutathione ABC transporter substrate-binding protein GsiB

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 517 signal peptide" amino acids 1 to 30 (30 residues), see Phobius details PF00496: SBP_bac_5" amino acids 73 to 431 (359 residues), 322.5 bits, see alignment E=2e-100

Best Hits

Swiss-Prot: 65% identical to GSIB_PECAS: Glutathione-binding protein GsiB (gsiB) from Pectobacterium atrosepticum (strain SCRI 1043 / ATCC BAA-672)

KEGG orthology group: K13889, glutathione transport system substrate-binding protein (inferred from 100% identity to rso:RSc1380)

MetaCyc: 62% identical to glutathione ABC transporter periplasmic binding protein (Escherichia coli K-12 substr. MG1655)
RXN0-11 [EC: 7.4.2.10]

Predicted SEED Role

"Dipeptide-binding ABC transporter, periplasmic substrate-binding component (TC 3.A.1.5.2); Putative hemin-binding lipoprotein" (TC 3.A.1.5.2)

Isozymes

No predicted isozymes

Use Curated BLAST to search for 7.4.2.10

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8XZL5 at UniProt or InterPro

Protein Sequence (517 amino acids)

>RS_RS06920 glutathione ABC transporter substrate-binding protein GsiB (Ralstonia solanacearum GMI1000)
MFNRFALGPRAAMLAGGLALAAFTMPAFAAKDAVMAVYSTFTTMDPYDANDTLSFSAAKS
FYQGLFGFDKDMKLVNVLCESYDVSKDGLVYTFRLHKGVKFHDGTTFDANAVKANLDRVT
NPANKLKRYTLFNRVAKTEVVDPYTARVTLKEPFSPFVNVLAHPSAAMISPAALQKYGKD
IAFHPVGTGPFEFVEWKQTDYLKGKKFAGYWKTGLPKIDTITWKPVVDNNTRATVMQTGE
ADFAFSIPFEQAAVLKGSPKVDLIAAPSIIQRYITFNTRVKPFDNPKVRQAINYAINKDA
LTKVAFSSYAVPADGVVPQGVDYAVKLGPWPYNPAKARALLKEAGYPDGFETQLWSAYNH
TTAQKIIQFVQQQLAQVGIKAQVQALEAGQRVERVESVQKPEDAGVRMYYMGWSSSTGES
DWAIRPLLASEAMPPKQLNTAYYKNDQVDADIAGALRTTDRAEKTKLYTDAQKRIWEDAP
WAFLVTEKVVYARAKRLSGAYVAPDGSFSFDDIDIKQ