Protein Info for RS_RS05670 in Ralstonia solanacearum GMI1000

Annotation: ferredoxin--NADP(+) reductase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 359 transmembrane" amino acids 30 to 41 (12 residues), see Phobius details PF12831: FAD_oxidored" amino acids 20 to 87 (68 residues), 27.7 bits, see alignment E=7e-10 PF07992: Pyr_redox_2" amino acids 20 to 311 (292 residues), 71.1 bits, see alignment E=4.3e-23 PF13450: NAD_binding_8" amino acids 23 to 86 (64 residues), 26.2 bits, see alignment E=3.1e-09 PF13738: Pyr_redox_3" amino acids 100 to 303 (204 residues), 47.1 bits, see alignment E=8e-16 PF00070: Pyr_redox" amino acids 171 to 211 (41 residues), 32.4 bits, see alignment 4.5e-11

Best Hits

Swiss-Prot: 100% identical to FENR_RALSO: Ferredoxin--NADP reductase (RSc1139) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K00384, thioredoxin reductase (NADPH) [EC: 1.8.1.9] (inferred from 100% identity to rso:RSc1139)

Predicted SEED Role

"Thioredoxin reductase (EC 1.8.1.9)" in subsystem Glycine reductase, sarcosine reductase and betaine reductase or Thioredoxin-disulfide reductase or Wyeosine-MimG Biosynthesis (EC 1.8.1.9)

MetaCyc Pathways

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 1.8.1.9

Use Curated BLAST to search for 1.8.1.9

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8Y0A5 at UniProt or InterPro

Protein Sequence (359 amino acids)

>RS_RS05670 ferredoxin--NADP(+) reductase (Ralstonia solanacearum GMI1000)
MTAETAFSAAPLPPVEQRIDVLIVGAGPVGLFAAFEAGVLGLSCVLVDVLDRPGGQCTEL
YPEKPIYDIPALVSCTAQELVDRLLAQCAPFGYPILCGRRAETVETIEGEHGRRFRVTTS
VGDVFDCAAVLITAGNGAFAPQRVALPEAAALEGRHLHYAVRDTARFAGKHVVVAGGGDS
ALDWALALRKTAARVTLVHRREGFRAADASVAGMRAAVAAGEMDFQVGMIGRLDSAPDGT
LTGIALRQREGETRLPCDELIALYGLVSEPGPIASWDVEMRAGRIVVETTAYETSRAGVF
AAGDIALYPNKQKLILSGFHEVAMALRRAYRYANPDKTLVHTHTSNDTNLQSRLHAAAE