Protein Info for RS_RS04990 in Ralstonia solanacearum GMI1000

Annotation: polyhydroxyalkanoate depolymerase

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 422 TIGR01849: polyhydroxyalkanoate depolymerase, intracellular" amino acids 2 to 412 (411 residues), 593.7 bits, see alignment E=8.1e-183 PF06850: PHB_depo_C" amino acids 211 to 413 (203 residues), 326.4 bits, see alignment E=3e-102

Best Hits

KEGG orthology group: K05973, poly(3-hydroxybutyrate) depolymerase [EC: 3.1.1.75] (inferred from 100% identity to rso:RSc1008)

Predicted SEED Role

"Intracellular PHB depolymerase (EC 3.1.1.-)" in subsystem Polyhydroxybutyrate metabolism (EC 3.1.1.-)

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.1.1.-

Use Curated BLAST to search for 3.1.1.- or 3.1.1.75

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8Y0N5 at UniProt or InterPro

Protein Sequence (422 amino acids)

>RS_RS04990 polyhydroxyalkanoate depolymerase (Ralstonia solanacearum GMI1000)
MLYQLHEFQRAMLSPMTAWAQATAKTFTNPLSPLSLLPGAQRYAAGYELLYRLGKEYEKP
EFNIRSVRSNGRDIPIVEQTIIEKPFCRLVRFKRYADDAETIRMLKDEPVVLVCAPLSGH
HSTLLRDTVRTLLQDHKVYVTDWLDARMVPVGAGPFHLSDYIAYIREFIAHIGADKLHVI
SVCQPTVPVLAAISLMASDGEKTPLTMTMMGGPIDARKSPTAVNSLATNKSHAWFENNVI
YTVPANYPGHGRRVYPGFLQHAGFVAMNPDRHVSSHYDYYLSLVEGDKEDAEAHVRFYDE
YNAVLDMAAEYYLDTIRDVFQEFHLANGTWVVDGKPVRPQDIKGTALFTIEGELDDISGS
GQTEAAHDLCSSIPKTRKQHLTASNCGHYGIFSGRRWREFVYPQLRDFIRRYDSSTRIKA
VA