Protein Info for RS_RS04840 in Ralstonia solanacearum GMI1000

Annotation: DNA topoisomerase IV subunit A

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 350 400 450 500 550 600 650 700 750 781 TIGR01062: DNA topoisomerase IV, A subunit" amino acids 15 to 712 (698 residues), 756.5 bits, see alignment E=1.3e-231 PF00521: DNA_topoisoIV" amino acids 38 to 496 (459 residues), 477 bits, see alignment E=5.6e-147 PF03989: DNA_gyraseA_C" amino acids 678 to 718 (41 residues), 24.2 bits, see alignment 1.8e-09

Best Hits

KEGG orthology group: K02621, topoisomerase IV subunit A [EC: 5.99.1.-] (inferred from 100% identity to rso:RSc0978)

Predicted SEED Role

"Topoisomerase IV subunit A (EC 5.99.1.-)" in subsystem DNA topoisomerases, Type II, ATP-dependent or Resistance to fluoroquinolones (EC 5.99.1.-)

Isozymes

Compare fitness of predicted isozymes for: 5.99.1.-

Use Curated BLAST to search for 5.99.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8Y0R5 at UniProt or InterPro

Protein Sequence (781 amino acids)

>RS_RS04840 DNA topoisomerase IV subunit A (Ralstonia solanacearum GMI1000)
MEQQDILFDPREPAEALTLAHYAERAYLDYAVSVVKGRALPEVADGQKPVQRRILFAMHE
MGLRADAKPVKSARVVGDVLGKYHPHGDQSAYDALVRLAQDFSLRYPLIDGQGNFGSRDG
DGAAAMRYTEARLTPISRLLLEELDQGTVDFIPNYDGSMDEPRLLPARLPFVLLNGASGI
AVGMATEIPSHNLREVATAAVALIRDDKLTSTDLMQYMPGPDFPGGGQIISSAADIAQIY
ETGRGSLKVRARWKIEDLARGQWQLVVTELPPNTSSQKVLEEIEELTNPKVRAGKKSLTP
EQLQLKQTLLGLLDAVRDESGKDAPVRLVFEPKSKNIGQQEFANALLAHTSLESGAAINL
VMIGTDGRPRQKGLREILREWIDYRFATVTRRTRHRLGKVEDRIHILEGRQLVLLNIDEV
IRIIRESDEPKPALMQAFGLSERQAEDILEIRLRQLARLEAIKIEQELKDLREEQAELDV
LLKSETMMRRRIVKEIETDAKQFGDDRRTLIQEERRAAAEVKVVDEPVTVIVSQKGWVRT
RQGHGHDATQFGFKAGDTLYDTFECRTTDALLIFGTRDDKGTGRVYSVAVANLPGGRGDG
VPLTTLIELAPGTHIAHTLASHAEQNVLISTQGGNGFLAKVGDMVGRQKGGKAFLTLDEG
DQPNKPAAVPEDAYGAACLSANGRLLLFPLAEVKTLASGGRGVILMELEKNETLAQALAF
GPAGFVVSGAGPRSGKATEQTFTTTRAQEPYAGKRARKGRAIEPKLRDPQLALPKPAPAA
A