Protein Info for RS_RS04675 in Ralstonia solanacearum GMI1000

Annotation: GTPase RsgA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 318 TIGR00157: ribosome small subunit-dependent GTPase A" amino acids 55 to 309 (255 residues), 213 bits, see alignment E=2.4e-67 PF03193: RsgA_GTPase" amino acids 74 to 251 (178 residues), 177.7 bits, see alignment E=9.9e-57

Best Hits

Swiss-Prot: 100% identical to RSGA_RALSO: Small ribosomal subunit biogenesis GTPase RsgA (rsgA) from Ralstonia solanacearum (strain GMI1000)

KEGG orthology group: K06949, ribosome biogenesis GTPase [EC: 3.6.1.-] (inferred from 100% identity to rso:RSc0940)

Predicted SEED Role

"Ribosome small subunit-stimulated GTPase EngC" in subsystem Universal GTPases

KEGG Metabolic Maps

Isozymes

Compare fitness of predicted isozymes for: 3.6.1.-

Use Curated BLAST to search for 3.6.1.-

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8Y0V3 at UniProt or InterPro

Protein Sequence (318 amino acids)

>RS_RS04675 GTPase RsgA (Ralstonia solanacearum GMI1000)
MTRGKPGRAGHDRRHASTGEHGLVIAAHGRHYLVERKGGGLLQCFPRGKRSECAVGDRVI
FEATAVDQGVVVRVEERRNLLHRSDQFKSKQLAANIDQVLIMLGTEPGFSEDLLGRALVA
AESLGITPLILLNKIDLTARLETARARLALYRALGYAVVELSVHAAPEAAHAVLAAHVAG
RSSILIGQSGMGKSSLLNLLIPGVDAQTREISEKLDSGKHTTTFTRLYHLPSGWGHGGTL
IDSPGFQEFGLHHLTEGMLERAFPEFRPRLTECRFYNCRHLQEPGCGILGAMAEGKIDPR
RHALYAQLLHESEQQKPW