Protein Info for RS_RS03695 in Ralstonia solanacearum GMI1000

Annotation: cell envelope integrity protein TolA

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 250 300 345 transmembrane" amino acids 20 to 39 (20 residues), see Phobius details TIGR02794: protein TolA" amino acids 19 to 342 (324 residues), 176.4 bits, see alignment E=1e-55 PF13103: TonB_2" amino acids 258 to 340 (83 residues), 62.4 bits, see alignment E=3.5e-21 TIGR01352: TonB family C-terminal domain" amino acids 284 to 342 (59 residues), 36.3 bits, see alignment E=5.7e-13 PF03544: TonB_C" amino acids 284 to 332 (49 residues), 27.4 bits, see alignment 3.8e-10

Best Hits

KEGG orthology group: K03646, colicin import membrane protein (inferred from 100% identity to rso:RSc0734)

Predicted SEED Role

"TolA protein" in subsystem Ton and Tol transport systems

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8Y1F6 at UniProt or InterPro

Protein Sequence (345 amino acids)

>RS_RS03695 cell envelope integrity protein TolA (Ralstonia solanacearum GMI1000)
MATTSLHRYEPVRERGTLRAFALAVLTHLLLLTFLYFGVQWQSSTPRGEEAELWDEAALQ
QAVQSAPAPEPKPEPAVQAPPAKVEEEADIALEQQKRKREEEAAAAREAELARRATAERE
DAKRQALLKEQKAQQARQAELQKRALAEQLAQQAKERQAHEKAVAAEAQARKDAQLKAQA
EAKAKAEAEARQKAKAVAEAKAKADAEQRAAQARSNAQRQARLRDLQALAGTAGATGAAA
GSGGGVGSGTGAGGTASAGYADRVRAKVQPNINFYDDTSGNPIVTVLVQMAPDGTVLSRR
VTKPSGYPVLDEAVLRAIDLSSPLPRDTNGKAPASVVVGFKPKRG