Protein Info for RS_RS03170 in Ralstonia solanacearum GMI1000

Annotation: hypothetical protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

Best Hits

KEGG orthology group: K01607, 4-carboxymuconolactone decarboxylase [EC: 4.1.1.44] (inferred from 100% identity to rso:RSc0631)

Predicted SEED Role

"4-carboxymuconolactone decarboxylase (EC 4.1.1.44)" in subsystem Protocatechuate branch of beta-ketoadipate pathway (EC 4.1.1.44)

MetaCyc Pathways

protocatechuate degradation II (ortho-cleavage pathway) (4/4 steps found)
aromatic compounds degradation via β-ketoadipate (6/9 steps found)
toluene degradation III (aerobic) (via p-cresol) (7/11 steps found)
superpathway of aerobic toluene degradation (13/30 steps found)
superpathway of aromatic compound degradation via 3-oxoadipate (15/35 steps found)

KEGG Metabolic Maps

Benzoate degradation via hydroxylation

Isozymes

Compare fitness of predicted isozymes for: 4.1.1.44

Use Curated BLAST to search for 4.1.1.44

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8Y1Q8 at UniProt or InterPro

Protein Sequence (242 amino acids)

>RS_RS03170 hypothetical protein (Ralstonia solanacearum GMI1000)
MNTSASSSTDPLFQMIGAVSPVLVPYTRHRIVEELWHRPGLQPRDRAIVTLSVLVSRNAS
NAYDHYLNKALDSGLKPSELSELLTHLAFYASFAYSFGAVAVLKGIFEARGITVDALPEV
SPALLAAEEALPGAAKRTAFLEANVAPASQALLHFTDALLNHEVWLRPGLATRDRALATI
ASLAAQGQADVLPTYLERALASGVTKEAIGELLAHVAFYGGWGNAIQAASAVSRYWSDSA
AG