Protein Info for RS_RS02025 in Ralstonia solanacearum GMI1000

Annotation: LPS export ABC transporter ATP-binding protein

These analyses and tools can help you predict a protein's function, but be skeptical. For enzymes, over 10% of annotations from KEGG or SEED are probably incorrect. For other types of proteins, the error rates may be much higher. MetaCyc and Swiss-Prot have low error rates, but the best hits in these databases are often quite distant, so this protein's function may not be the same. TIGRFam has low error rates. Finally, many experimentally-characterized proteins are not in any of these databases. To find relevant papers, use PaperBLAST.

Protein Families and Features

1 50 100 150 200 262 signal peptide" amino acids 1 to 19 (19 residues), see Phobius details TIGR04406: LPS export ABC transporter ATP-binding protein" amino acids 20 to 262 (243 residues), 387.7 bits, see alignment E=8.2e-121 PF00005: ABC_tran" amino acids 36 to 187 (152 residues), 121.9 bits, see alignment E=3.2e-39 PF12399: BCA_ABC_TP_C" amino acids 236 to 259 (24 residues), 27.5 bits, see alignment (E = 1.9e-10)

Best Hits

Swiss-Prot: 58% identical to LPTB_ECOL6: Lipopolysaccharide export system ATP-binding protein LptB (lptB) from Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)

KEGG orthology group: K06861, lipopolysaccharide export system ATP-binding protein [EC: 3.6.3.-] (inferred from 100% identity to rso:RSc0409)

MetaCyc: 58% identical to lipopolysaccharide transport system ATP binding protein LptB (Escherichia coli K-12 substr. MG1655)
TRANS-RXN-237 [EC: 7.5.2.5]

Predicted SEED Role

"Lipopolysaccharide ABC transporter, ATP-binding protein LptB" in subsystem KDO2-Lipid A biosynthesis

Isozymes

Compare fitness of predicted isozymes for: 3.6.3.-

Use Curated BLAST to search for 3.6.3.- or 7.5.2.5

Sequence Analysis Tools

PaperBLAST (search for papers about homologs of this protein)

Search CDD (the Conserved Domains Database, which includes COG and superfam)

Search structures

Predict protein localization: PSORTb (Gram-negative bacteria)

Predict transmembrane helices and signal peptides: Phobius

Check the current SEED with FIGfam search

Find homologs in fast.genomics or the ENIGMA genome browser

See Q8Y2C7 at UniProt or InterPro

Protein Sequence (262 amino acids)

>RS_RS02025 LPS export ABC transporter ATP-binding protein (Ralstonia solanacearum GMI1000)
MSATAQATATIAPQAATSSTLVVRHLKKRYGTRTVVKDVSLDVKSGEVVGLLGPNGAGKT
TSFYMIVGLVALDAGDIVLDGQHISRLPIHQRARMGLSYLPQEASVFRKLNVQENIRAVL
ELQEQDGKPLSSTEVNRRLDALLDDLQIAHLRDNPALSLSGGERRRVEIARALASSPRFI
LLDEPFAGVDPIAVGEIQRIVSFLKDRNIGVLITDHNVRETLGICDHAYIISEGTVLAAG
MPEQIVENEDVRKVYLGDNFRM